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Choose Enzymes Manually

Choose enzymes manually by name, or by editing an existing enzyme set or using search criteria.

Choose an Enzyme Set

To choose an existing enzyme set, click Enzymes → Choose Enzymes, then select the enzyme set name in the Choose from list.

Choose an Enzyme by Name

To search within the chosen enzyme set, enter the enzyme name in the search box.

Alternatively, click on an enzyme name to select it.

Add to an Enzyme Set

To add the selected enzyme(s) to the Chosen Enzymes set, click the Add → button.

To add all of the enzymes from the original set to the Chosen Enzymes set, click the Add All → button.

Remove from an Enzyme Set

To remove the selected enzyme from the Chosen Enzymes set, click the Remove button or press the Delete key.

To remove all of the enzymes from the Chosen Enzymes set, click the Remove All button.

Choose Enzymes by Criteria

To choose enzymes based on criteria, click the Choose from check box. Enzymes that meet the selected criteria will be highlighted in the Choose from list.

To specify the enzyme set from which the enzymes will be chosen, click the Choose from menu button.

To specify where the chosen enzymes will cut, click the Enzymes cut menu button.

To specify the number of cut sites, type a number in the selected box. If not previously specified, the cut site region can be specified on the right.

To specify the enzyme overhang type, click the Overhang menu button.

To specify the recognition sequence length, click the Recognition Sequence menu buttons.

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