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Annotate an Intron

How can I add an intron annotation to a translated feature?

View the Translated Feature

In this example, a Saccharomyces cerevisiae tubulin gene has been annotated as a translated feature. The intron has not yet been specified, and as a result, the translation contains stop codons.

Select the Intron Sequence

According to the Saccharomyces Genome Database, the intron lies between bases 26 - 141. To specify such a numerical range click Edit → Select Range... , type the range, then click Select.

Delete the Selected Feature Segment

To remove the intron from the feature, click Features → Delete Feature Segment....

When the Edit Feature dialog appears, click OK.

View the Intron

A dashed line will now mark the intron. The translation will adjust to span the intron boundaries. In this example, the codon for Valine-9 is split between the two exons.

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