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Restriction Cloning and Linear Ligation

This set of lessons describes how to clone and ligate restriction fragments.

Insert Fragment
How can I insert a restriction fragment into a circular vector?

Insert Multiple Fragments
How can I insert multiple restriction fragments into a circular vector?

Delete Restriction Fragment
How can I delete an internal restriction fragment in a circular vector?

Destroy Restriction Site
How can I destroy a restriction site in a circular plasmid?

Linear Ligation
How can I ligate two or more linear fragments?

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