Home » Release Notes » 5.1.0

Changes in version 5.1 (Apr 13, 2020)

New Functionality

  • Enabled DNA and protein sequence windows to be split to show two views, one above the other.
    (Requested over the years by multiple users)
  • Added support in the Description Panel for various reference types including books, database entries, patents (which can be imported from online databases), theses, web links, and more.
  • Configured /citation qualifier values to link to specific references, which are accessible via tooltips and clickable links in Features view.
  • Added optional page number entries for /citation qualifiers.
  • Enabled enzyme visibility to be adjusted in Enzymes view using check boxes, mirroring the controls used for Features and Primers views.
  • Enabled flexible feature detection with an adjustable similarity threshold when importing features from another file.
  • Added support for exporting aligned sequence trace data using a tab-separated format.
    (Requested by Yves Girerd-Chambaz)
  • Enabled BLAST for multiple selected sequence files in a collection.
    (Requested by Charles Dozois)
  • Added a pre-defined enzyme set for Type IIS enzymes.
  • Enabled import of features or primers from another file, or detection of common features, for multiple selected DNA files in a collection.
    (Requested by Michael Gotrik)
  • Enabled simultaneous flipping of multiple selected DNA sequences in a collection.
  • Added support for linking folders of the same name in different areas of a collection, with "Go to ..." shortcuts for switching between related folders in different areas.
    (Requested by Mily Ron)
  • Enabled the "Make Protein" and "Reverse Translate" commands, when operating within a collection, to save the output files to a linked folder in a different area of the collection.
  • Added support for batch replacement of Description Panel fields for files in a collection.
    (Requested by Samuel Tremblay-Belzile)
  • Added MW markers from Nacalai Tesque.
    (Requested by Tomonori Hoshino)
  • Added MW markers from FroggaBio.
    (Requested by Michael Ostrovski)
  • Added MW markers from Newmarket Scientific.
    (Requested by Sabrina)
  • Added the remaining TrackIt™ MW markers from Thermo Fisher.
    (Requested by Slava Gurevich)
  • Improved feature tooltips when using compact mode in Sequence view.
    (Requested by Bandar)


  • Reduced the height of linear maps by enabling items to be superimposed without sacrificing legibility.
    (Requested by Vishal Chaudhari)
  • Changed the defaults to show HF® and FastDigest® information in the Restriction Enzymes dialog, while allowing these defaults to be adjusted.
    (Requested by Silvana Jirka)
  • Enhanced the File > Export cascading menu to enable export of multiple files or a list of files from a collection.
    (Requested by Gurumoorthy Krishnamoorthy)
  • Added support for a color attribute when importing features from GFF3 format.
    (Requested by Matthew Kirk)
  • Added support for specifying the line break characters when exporting to text file formats.
    (Requested by Tim Fallon and Tom Chappell)
  • Enabled long map labels to span two lines in circular Map view.
    (Requested by Chun Cao)
  • Configured Sequence view to retain visibility of the scrolled region when toggling horizontal scrolling.
    (Requested by Kengo Adachi)
  • Enhanced the flexibility of code number display in collection lists.
    (Requested by Tim Nierhaus)
  • Added a keyboard shortcut for "Import UniProt Sequences".
    (Requested by James Wright)
  • Enabled colors in multiple sequence alignments to be copied to the clipboard.
    (Requested by Monir Ejemel)
  • Added support for importing Vector NTI Express Designer databases
    (Requested by Dave Karig)
  • Provided the option to differentiate "A" traces using a striped pattern for users with color vision disabilities.
  • Provided an adjustable similarity threshold for detecting common features.
  • Enabled the "Make Protein", "Reverse Translate", and "New File From Selection" commands to save the output files to the same collection.
  • Allowed the genetic code to be specified when converting a selection in a DNA alignment to a protein alignment.
  • Ensured that when sorting by Code Number or Alias in a collection, any files with blank entries are placed at the end of the list.
  • Converted SnapGene to a 64-bit application on Windows, thereby improving performance and stability.
  • Ensured that scrollbars are shown only when necessary in Map, Sequence, and History views.
  • Improved the wording in the Restriction Enzymes dialog to make it clearer when a supplier offers multiple variants of an enzyme.
  • Changed the License Agreement dialog so that it is no longer modal, and added support for printing or saving this document using keyboard shortcuts.
  • Made various textual, color, and icon enhancements.


  • Configured the Find control to show bottom strand results when using compact format in Sequence view.
    (Requested by Bandar)
  • Prevented extra line breaks from appearing after export of some qualifier values to GenBank and EMBL formats.
    (Reported by Karen Ross)
  • Ensured that all controls in alignment dialogs can be translated into Japanese or Chinese.
  • Ensured that collection folder names for selected items never appear in List view.
  • Enabled scrolling up using Shift + Spacebar in Sequence view.
  • Fixed paging up and down using Function + Up/Down keys, and scrolling to the start or end of the sequence using Function + Left/Right keys.
  • Ensured that ancestral blunt cutters can be shown in green in History view after import from Vector NTI® or other file formats.
  • Prevented unnecessarily asking to add methylation when specifying "Escherichia coli" as the Source for a Natural DNA sequence.
  • Corrected an error with internal array data when editing trace sequences.
  • Ensured that linearizing or circularizing a DNA sequence populates the Sequence Author field with the default author.
  • Configured Enzymes view to show enzyme sites in red upon mouseover in both the main view and the lower map.
  • Corrected codon frequencies for reverse directional ORFs.
  • Ensured that the Description Panel flows to the bottom of the window when zoom controls are shown.
  • Fixed various issues with importing primers into DNA files in a collection.
  • Ensured that the map label in a circular map never overlaps features.
  • Disable menu File > Export > History when no history exists in sequence file.
  • Fix Blasting selected primer in a collection file.
  • Fix Tools > Blast Selected Protein with a collection file.
  • Ensured that the Previous button in the Find controls is shown in blue only if the Shift key and no other key is pressed.
  • Adjusted the behavior for sequence traces so that mousing over an ambiguous base darkens the curves for multiple bases.
  • Prevented accented characters from being accidentally inserted into a DNA or protein sequence when holding down letter keys on macOS.
  • Improved the foreground color within features while using compact Sequence view.
  • Improved feature pliancy while using compact Sequence view.
  • Fixed an issue in which pressing the Shift key resulted in a button changing color in the "Import Features from a SnapGene File" and "Detect Common Features" dialogs.
  • Improved the look and feel of controls for Enzymes view on Windows.
  • Preserved formatting when creating hyperlinks.
  • Improved horizontal alignment of values shown in the Properties tab for protein sequences.
  • Enabled proper export of /citation qualifier values to GenBank, GenPept, and EMBL formats.
  • Ensured that suppliers remain selected after dragging them in the Enzymes tab of the Preferences dialog.
  • Updated the MW calculation for a full sequence in the DNA Calculations window after 5' phosphates are added or removed.
  • Enabled bottom strand matches to search queries in Sequence view when using compact mode.
  • Ensured that qualifier selections in Features view are cleared when selecting features or transferring focus to the Description Panel.
  • Configured a collection to show and select imported files even when those files are not in the currently chosen category.
  • Fixed an issue that could result in labels for Site features not being shown entirely within a protein sequence window.
  • Improved the horizontal placement of names and connecting lines for site features in Sequence view.
  • Fixed an issue in which selections in Features and Primers views sometimes remained blue after focus was transferred to the Description Panel.
  • Disabled controls for setting the DNA color when using either compact Sequence view or Map view with features on the DNA line.
  • Fixed issues with displaying enzymes that make two double-stranded cuts when a site is near the end of a linear sequence.
  • Improved stability when importing Vector NTI Express Designer enzyme names
    (Reported by Dave Karig)