Home » Release Notes » 5.1.4

Changes in version 5.1.4 (Jun 17, 2020)

New Functionality

  • Added support for importing protein features from the GFF3 format.
    (Requested by Takeda)
  • Enabled features to be preserved when a replacement leaves the sequence length unchanged.
    (Requested by Sanofi)


  • Enabled the import of custom user fields from Vector NTI databases.
    (Requested by Decheng)
  • Increased the 3' match length limit to 25 bases when importing primers from a list.
    (Requested by Yield10 Bio)
  • Enabled capture of the history of a protein translated from DNA when importing from Vector NTI Advance.
    (Requested by Romain Merceron)
  • Enhanced primer tooltips to include % GC for the annealed region.
    (Requested by Ori Mayer)
  • Improved copy and paste of sequence alignments into text editors and other programs.
    (Requested by Pam Cook)
  • Increased the size of the DOI field when editing references.
  • Made various color, layout, and textual enhancements.


  • Mandated use of the proper default font size when creating new files, importing from online, and opening non-native files.
    (Reported by Donelson Smith)
  • Corrected an issue that prevented importing primers into multiple files in a collection.
    (Reported by Michael Brasino)
  • Corrected a regression with displaying translations and the "Original Sequence" when no sequences are aligned to the reference DNA sequence.
    (Reported by Scott James)
  • Enabled changing methylation for placeholder files.
  • Fixed various issues when working with placeholder files.
  • Implemented automatic correction of invalid alignments to a reference sequence computed with prior versions.
  • Fixed an issue that prevented manually specifying the zoomed range.
  • Corrected the "+" symbol in plasmid names when importing from "SnapGene Online Sequences".
  • Ensured correct updating of the zoomed range when navigating to matches with the Find tool.
  • Addressed an issue with specifying the position of protein interchain bond locations.
  • Removed the "Codons" cascading menu when interacting with protein sequences.
  • Corrected an issue that prevented importing multi-sequence GenBank and GenPept files into a collection.
  • Ensured that translation numbering is maintained when using Make Protein from multiple selected translated features.
  • Ensured that the match threshold controls are listed only once when importing features from a SnapGene file.
  • Corrected an issue that could cause file names to be clipped in source menus.
  • Fixed an issue that caused files to be marked as modified after hovering over hyperlinks in the Description Panel.
  • Ensured that stop codons present in protein query strings are included in the search results.
  • Improved the import of multi-part qualifiers from Geneious.
  • Fixed an issue with undo of sequence color changes that are limited to a single strand.
  • Corrected the "Copy" shortcut when viewing a protein sequence or multi-protein alignment.
  • Ensured more robust behavior when adding primers to DNA sequences.
  • Turned on the display of truncated primer description data when full descriptions are toggled off in Primers view.
  • Corrected an issue in which simplified primer binding sites were not shown after modifying hybridization parameters.
  • Ensured that sufficient space is always allocated above displays of complex primer binding sites in Sequence view.
  • Turned on display of the parental enzyme set when viewing Nicking Endonucleases in Sequence and Map views.
  • Improved reliability when importing from Addgene.
  • Improved the import of Addgene sequences that contain slashes in their names.
  • Ensured that side toolbar buttons in dialogs are not hidden.
  • Corrected a regression in which two copies of common features such as EGFP were sometimes annotated when detecting common features.
  • Removed "Match Threshold" controls from the "Add to Common Features" dialog.