RNA structures can be recalculated using adjusted Tm and other settings
Display and make sequence selections in Structure view
Coordinates and 5' / 3' end labels can optionally be displayed in Structure view
Added menu actions at the top right of Structure view to reset pane, rotation and scale
Added a ligation fidelity matrix to the Golden Gate Assembly tool for assessing overall reaction fidelity
Cut a Golden Gate Assembly vector with any enzyme (not just Type IIS enzymes)
Adjust overhangs for manually designed primers while simulating Golden Gate Assembly
Adjust the hybridization region for all PCR cloning simulations. When using automatic primer design, the
hybridized region will be configured automatically, ensuring miscellaneous features are not transferred to the product
when a 5’ primer extension by chance partially hybridizes to the template.
Copy or export the properties and amino acid data for full protein sequences or selected regions
Copy individual protein properties
Check for and prevent simulating Golden Gate assembly if two adjacent fragments are set to be used directly and
abutting ends both lack 5' terminal phosphates
Improved the appearance of many icons on low DPI displays
Display the selected MW in the selection bar for protein alignments
Improved the error message shown when attempting to ligate linear fragments that lack 5' terminal phosphates
Updated the Golden Gate Assembly dialog to include easy access to recommended enzymes
Improved the default sequence name when pasting FASTA encoded sequences into the New File dialog
Modernized application, file, and various other icons
Added links to NEBuilder® HiFi DNA Assembly and TOPO® Cloning tutorial videos.
Show complementary bases of neighboring overhangs in Golden Gate cloning mini-overviews
Zoom to fit by default when viewing RNA secondary structures
Fixed an issue where quality of sequences aligned to a reference was sometimes not shown when requested
Improved the appearance of tab controls on macOS
Improved the appearance of slider controls on macOS and fixed an issue that prevented vertically scaling peaks
for traces aligned to a reference sequence
Improved overall stability
Fixed an issue that prevented simulating Golden Gate cloning when a golden gate site blocked by methylation was
present within the insert or in the assembled product
Corrected an issue where when exporting translated features that use a custom genetic code (e.g. Amber) to GenBank
the /transl_table qualifier should be omitted and this information is encoded using a /note qualifier instead
Fixed issues with importing multi-sequence GenBank files that contain empty sequences (name only)
Fixed an issue where protein sequences were sometimes imported from NCBI as DNA or RNA when using the import
extra features option
Fixed issues with selecting the codons just upstream and downstream of the site of ribosomal slippage
Fixed an issue that sometimes prevented aligning bases adjacent to internal mismatching regions
Ensure "Show History" is always a blue clickable link in textual representation of History view
Corrected the default methylation for newly created sequences to match the default strain specified in Preferences
Disabled the 3-Letter Amino Acids action in the sequence view context menu when using compact mode
Fixed a crash when detecting common features
Fixed an issue where quality data was sometimes not shown for all sequences aligned to a reference
Fixed an issue with stripping out some formatting when copy and pasting from web pages into the description panel or
other rich text controls
Corrected an issue where feature colors were not accurately shown when using dark mode
Addressed a number of issues with creating features in protein sequences
Only allow a single copy of the alignment dialogs to be shown at a time
Fixed the horizontal alignment of the "Show as Uninterrupted Circle" button in the side toolbar
Do not show a cursor when clicking and holding on a codon in Sequence view
Avoid showing duplicate copies of open documents from the Window menu
Do not show a translation window when starting SnapGene on a computer configured to use a non-English locale such as
German but one that the application is not translated into
Removed duplicate "site" feature type from the feature type cascading menu
Improve area printed when printing RNA structures
Fixed an issue where printed RNA structures were sometimes blurry
Fixed an issue where an I-beam cursor was shown when mousing over Sequence view in contexts where arbitrary selections
cannot be made such as the Silent Mutagenesis dialog
Fixed an issue that prevented detecting the preferred language on macOS
Fixed an issue where the wrong number of binding sites was sometimes listed in cloning dilaogs when simplified
binding sites were shown
Fixed an issue where primers designed automatically and those specified manually were not always shown annealing
to the template in the same manner
Fixed various issues when using multiple screens.
Various textual corrections
Fixed a stability issue when attempting to use a linear fragment directly when simulating Overlap Extension PCR
Fixed an issue where the application would freeze when attempting to generate primers for Golden Gate cloning if the
fragments already contained one or more sites for the Golden Gate enzyme.
Fixed issues where windows were not always shown on the logical display when using multiple displays on macOS.
Improved opening older Geneious files.
Fixed an issue where alternate transcripts (isoforms) were incorectly listed already in and thus not imported directly
when importing features from another file.
Fixed an issue that could prevent batch importing features
Fixed an issue where when importing an enzyme list unknown entries were imported as AanI.
Fixed an issue where using the Choose Primers command would fail to design primers for linear vectors or fragments if
you did not first make a selection. SnapGene now correctly designers primers to use the entire linear sequence.
Fixed a stability issue when changing the number of fragments to 1.
Fixed an issue where if the first or last fragment in Overalap Extension PCR was not amplified by PCR the forward
and/or reverse primers for amplifying the assembled linear fragment were not configured automatically when using the
Choose Primers command.
Fixed ordering from Vector Builder
Fixed the link for more information about fonts and printing on Windows
Improved rendering buttons and images when dragging windows between low and high resolution displays
Fixed various memory leaks
Fixed an issue where primers binding sites with melting temperatures less than 30 C were not identified
Improved the behavior of the Secondary Structure zoom control
Improved decoding feature types from Vector NTI databases
Removed the MAFFT penalty shift setting since it was problematic
Updated links to the user guide and user guide articles