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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Zhang lab plasmid for expressing a chimeric guide RNA (gRNA) plus a puromycin resistance marker and human codon-optimized Cas9 with the D10A nickase mutation.

 

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pSpCas9n(BB)-2A-Puro (PX462) V2.0.dna

Map and Sequence File:    Download    Open    

Sequence Author:  Zhang Lab / Addgene #62987

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