hmKO2

Humanized monomeric Kusabira-Orange 2 fluorescent protein.

Sequence Author: MBL International

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No matches
600 400 200 End (657) BtgI (641) BsaHI (638) TaqII (622) BfuAI - PaqCI - BspMI (618) PfoI * (557) XcmI (555) StyI (548) BmgBI (477) BmrI (432) BanII (431) AleI - MslI (373) BsrFI - SgrAI (370) BglI (338) HaeII (323) EciI (297) PpuMI (281) EcoNI (276) StuI (253) EarI (221) PmlI - BsaAI (186) AflIII (185) AhdI (177) CsiI - SexAI * (173) NmeAIII (167) BtsI - BtsαI (124) BaeGI - Bme1580I (91) DrdI (52) AvaI - BsoBI (19) BclI * (11) Start (0) hmKO2 hmKO2 657 bp
End  (657)
0 sites
BtgI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaHI  (638)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
TaqII  (622)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BfuAI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
PaqCI  (618)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BspMI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PfoI  (557)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
XcmI  (555)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
StyI  (548)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BmgBI  (477)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BmrI  (432)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BanII  (431)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
AleI  (373)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (373)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsrFI  (370)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (370)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BglI  (338)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
HaeII  (323)
1 site
R G C G C Y Y C G C G R
EciI  (297)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
PpuMI  (281)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoNI  (276)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
StuI  (253)
1 site
A G G C C T T C C G G A
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PmlI  (186)
1 site
C A C G T G G T G C A C
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
AflIII  (185)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
CsiI  (173)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (173)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NmeAIII  (167)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtsI  (124)
1 site
G C A G T G N N C G T C A C
BtsαI  (124)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BaeGI  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AvaI  (19)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (19)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hmKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: humanized monomeric Kusabira-Orange 2 fluorescent protein
mammalian codon-optimized
hmKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: humanized monomeric Kusabira-Orange 2 fluorescent protein
mammalian codon-optimized
ORF:  1 .. 657  =  657 bp
ORF:  218 amino acids  =  24.5 kDa
ORF:  1 .. 657  =  657 bp
ORF:  219 amino acids  =  22.3 kDa  (no start codon)
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