pAAV-AC-Myc-DDK

Adeno-associated virus (AAV) vector for expressing a C-terminally myc- and FLAG-tagged protein using the CMV promoter.

Sequence Author: OriGene

|Download SnapGene Viewer
No matches
PciI (4749) NmeAIII (3714) FspI (3638) ScaI (3380) XmnI (3261) PsiI (2590) DraIII (2465) NdeI (390) SnaBI (496) NcoI (516) Eco53kI (722) SacI (724) SacII (811) BsaBI (817) BGI-F sequencing primer (1173 .. 1192) BspDI * - ClaI * (1317) BseRI (1339) AsiSI (1344) MreI - SgrAI (1346) AscI (1350) HindIII (1364) NheI (1375) BmtI (1379) MluI (1387) BsiWI (1390) PaeR7I - PspXI - XhoI (1402) EcoRV (1446) PmeI (1480) FseI (1490) AleI (1558) XL39-R sequencing primer (1564 .. 1583) BsgI (1742) BbvCI - Bpu10I (1784) SphI (1817) NsiI (1819) BlpI (1828) BstEII (1989) PmlI (1997) KasI (2158) NarI (2159) SfoI (2160) PluTI (2162) pAAV-AC-Myc-DDK 4754 bp
PciI  (4749)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
NmeAIII  (3714)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FspI  (3638)
1 site
T G C G C A A C G C G T
ScaI  (3380)
1 site
A G T A C T T C A T G A
XmnI  (3261)
1 site
G A A N N N N T T C C T T N N N N A A G
PsiI  (2590)
1 site
T T A T A A A A T A T T
DraIII  (2465)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NdeI  (390)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (496)
1 site
T A C G T A A T G C A T
NcoI  (516)
1 site
C C A T G G G G T A C C
Eco53kI  (722)
1 site
G A G C T C C T C G A G
SacI  (724)
1 site
G A G C T C C T C G A G
SacII  (811)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsaBI  (817)
1 site
G A T N N N N A T C C T A N N N N T A G
BspDI  (1317)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1317)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BseRI  (1339)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AsiSI  (1344)
1 site
G C G A T C G C C G C T A G C G
MreI  (1346)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (1346)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1350)
1 site
G G C G C G C C C C G C G C G G
HindIII  (1364)
1 site
A A G C T T T T C G A A
NheI  (1375)
1 site
G C T A G C C G A T C G
BmtI  (1379)
1 site
G C T A G C C G A T C G
MluI  (1387)
1 site
A C G C G T T G C G C A
BsiWI  (1390)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
PaeR7I  (1402)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1402)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1402)
1 site
C T C G A G G A G C T C
EcoRV  (1446)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PmeI  (1480)
1 site
G T T T A A A C C A A A T T T G
FseI  (1490)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
AleI  (1558)
1 site
C A C N N N N G T G G T G N N N N C A C
BsgI  (1742)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbvCI  (1784)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1784)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
SphI  (1817)
1 site
G C A T G C C G T A C G
NsiI  (1819)
1 site
A T G C A T T A C G T A
BlpI  (1828)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BstEII  (1989)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PmlI  (1997)
1 site
C A C G T G G T G C A C
KasI  (2158)
1 site
G G C G C C C C G C G G
NarI  (2159)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (2160)
1 site
G G C G C C C C G C G G
PluTI  (2162)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BGI-F sequencing primer
20-mer  /  45% GC
1 binding site
1173 .. 1192  =  20 annealed bases
Tm  =  53°C
XL39-R sequencing primer
20-mer  /  55% GC
1 binding site
1564 .. 1583  =  20 annealed bases
Tm  =  59°C
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3074 .. 3142  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3143 .. 3934  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4105 .. 4693  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4105 .. 4693  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
1501 .. 1977  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
1501 .. 1977  =  477 bp
human growth hormone polyadenylation signal
f1 ori
2232 .. 2687  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2232 .. 2687  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
chimeric intron
873 .. 1251  =  379 bp
fusion between human cytomegalovirus intron A and human β-globin intron 2
chimeric intron
873 .. 1251  =  379 bp
fusion between human cytomegalovirus intron A and human β-globin intron 2
CMV enhancer
217 .. 520  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
217 .. 520  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
521 .. 724  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
521 .. 724  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2017 .. 2157  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2017 .. 2157  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
2969 .. 3073  =  105 bp
AmpR promoter
2969 .. 3073  =  105 bp
MCS
1324 .. 1408  =  85 bp
multiple cloning site
MCS
1324 .. 1408  =  85 bp
multiple cloning site
Myc
1405 .. 1434  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
1405 .. 1434  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
FLAG
1453 .. 1476  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
1453 .. 1476  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
ORF:  3074 .. 3934  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1815 .. 2255  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  1865 .. 2212  =  348 bp
ORF:  115 amino acids  =  13.4 kDa
ORF:  3538 .. 3804  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pAAV-AC-Myc-DDK.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.