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SnapGene®

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System Requirements
OS Windows 7 or later
macOS 10.10 or later
Fedora Linux 21 or later
Red Hat Linux 7.2 or later
Ubuntu Linux 14.04 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Changes in version 3.1 (Mar 9, 2016)

New Functionality

  • A compact Sequence view mode.
  • Option to display features on the DNA map line.
  • Support for NEBuilder® HiFi DNA Assembly.
    (Requested by Benjamin Spreng, Qiyu Zhang, and Monika Dieterle)
  • Ability to regenerate individual restriction sites in the vector when designing primers for Gibson Assembly® and In-Fusion® cloning.
    (Requested by John Murray)
  • Importer for DS Gene files.
    (Requested by Marie-Ève Bouthot)
  • Importer for GeneTool files.
    (Requested by Wolfgang Strobel)

Enhancements

  • Enabled Gibson Assembly® of linear fragments to generate a circular product.
    (Requested by Guy Mikawa)
  • Added Lucigen DNA ladders.
    (Requested by Ron Godiska)
  • Added pcDNA3.2/capTEV-NT/V5-DEST and pcDNA3.2/capTEV-CT/V5-DEST Gateway® Destination vectors.
    (Requested by Maria Landrock)
  • Ensured that SnapGene is offered as an option in the "Open With" context menu on Windows.
    (Requested by Philipp Glock)
  • Added a draggable splitter to the list in the Browse Common Features dialog.
    (Requested by Wulf Dirk Leuschner)
  • Added a check box to the Browse Common Features dialog to make it easier to enable detecting a translated feature by its protein sequence.
    (Requested by Wulf Dirk Leuschner and Mathias Mahn)
  • Added a keyboard shortcut for "Copy Protein”.
    (Requested by Scott James)
  • Configured the "Set DNA/Protein Color" dialogs to remember the most recently used color and strands.
    (Requested by Seth Goldman)
  • Added a check so that if a newly created feature will not be shown in the circular map without prioritization, SnapGene offers to prioritize it.
  • Improved how multiple adjacent copies of the same feature are labeled in maps.
  • Ensured that if enzymes and primers (DNA) or regions (Protein) are toggled off, the locations/numbers button in the side toolbar is disabled.
  • Added a "Trim Selected Region" command to the context menu when right clicking aligned sequences in Sequence view.
  • Added the Cmd+Backspace shortcut on OS X for discarding unsaved changes.
  • Added "lncRNA" and "ribozyme" to the list of predefined /ncRNA_class qualifier values.
  • Added "Gen9" to the list of sequence authors.
  • Sped up scrolling in Sequence view while the minimap is visible.
  • Sped up loading and displaying circular maps in action dialogs.
  • Enhanced the Import from GenBank dialog to support importing the antisense strand by using the "complement" keyword.
  • Implemented various color, textual, and alignment enhancements and other optimizations.

Fixes

  • Removed a duplicate "Enter/Exit Full Screen" command from the View menu on OS X 10.11.
  • Fixed an unlikely issue that could result in truncating some Jellyfish, Visual Cloning, and DNADynamo files.
  • Fixed an issue where custom primers for PCR were not migrated to the amplified product if their names were edited by copying and pasting.
    (Reported by Michael @ Novozymes)
  • Enabled the detection of attB1.1 and attB2.1 Gateway® cloning sites.
    (Reported by Max Schnepf)
  • Ensured that newly saved files use default attributes for the directory in which they are saved on Windows.
    (Reported by Brian Ayre)
  • Improved the display for a reverse directional primer with a 5' tail extending beyond the end of the sequence when using horizontal scrolling.
    (Reported by Hugo de Jonge)
  • Fixed an issue where the wrong tab was sometimes shown when clicking a link to pull up the relevant setting in the Preferences dialog.
    (Reported by Wulf Dirk Leuschner)
  • Improved the agarose gel display when using a Japanese translation.
    (Reported by Yusuke Miyanari)
  • Enabled the import of protein sequences with accession numbers for PDB structures.
    (Reported by Jim Meador)
  • Enhanced the EMBL file importer to recognize files exported from Vector NTI.
    (Reported by Markiyan)
  • Fixed an issue with displaying enzyme, feature, and primer labels on the right side of some circular maps.
    (Reported by John Murray)
  • Fixed an issue where the number of mismatches in an alignment was sometimes printed incorrectly.
    (Reported by Gabriele Kleiner)
  • Fixed issues with exporting dates in EMBL/GenBank format.
  • Prevented invalid characters from being included before sequence data when opening EMBL or GenBank files that include a comment on the ORIGIN line.
  • Improved decoding of the code number when opening EMBL files.
  • Enhanced the GenBank importer to tolerate "pb" as the unit length in the LOCUS line.
  • Enhanced the GenBank importer to ignore empty fields marked with the “/“ character.
  • Embedded application information and a manifest for Windows, and code signed all .exe and .dll files.
  • Configured the label controls in the side toolbar and Map & Sequence Options dialog to be disabled if features/regions are toggled off.
  • Fixed an issue where the DNA tab of the Preferences dialog would not list newly saved custom enzyme sets while the dialog was open.
  • Fixed an issue that could result in a hang.
  • Removed Pyl, Sec, and Xle from the “Insert Codon” controls in the Add/Edit Primer dialogs, and added TAA (Ochre), TAG (Amber), and TGA (Opal) to the list of possible codons when inserting Xaa.
  • Fixed issues with showing the genetic code in the Add/Edit Feature dialogs and Features view when Ochre, Amber, or Opal were used.
  • Improved feature and ORF tooltip placement when the tooltip could not be shown in the preferred location outside a circular map.
  • Ensured that regions of overlap are properly highlighted in black in History view for fragments that are flipped while performing Gibson Assembly® or In-Fusion® cloning.
  • Ensured that after simulating Gibson Assembly® or In-Fusion® cloning, portions of fragments or the vector that were trimmed away are highlighted in white in History view.
  • Fixed issues with opening DNADynamo and Jellyfish protein files.
  • Improved the display of certain feature labels above a linear map.
  • Fixed an issue that prevented software update from working for SnapGene Viewer on Mac OS X. -Fixed an issue with detecting "misc_RNA" and other feature types for which a single nucleotide can be omitted from either end.
  • Fixed an issue where "Nonstandard genetic code" was unnecessarily displayed in the "Edit Feature" dialog after making a feature from an aligned sequence.

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