Explore our educational resources to advance your knowledge of SnapGene, cloning and core molecular biology procedures.

Getting Started

See how SnapGene works. Learn to plan, visualize and document molecular cloning procedures.
Learning how to use SnapGene is easy! We developed a few short videos to get you started.
If you're new to SnapGene this brief guide will help you get started.

Using SnapGene

Watch our easy to follow videos and learn how annotate features, create primers, simulate PCR and perform silent mutations
Learn how to verify your cloning and validate your experiments with tools including alignments and agarose gel simulations.
Learn how to simulate restriction cloning in SnapGene with this step-by-step walkthrough video series.
Learn how to simulate TOPO cloning in SnapGene with this step-by-step video tutorial.
Learn how to simulate TA and GC cloning in SnapGene, with this video series.
Learn all the steps needed to simulate single or multisite Gateway Cloning in SnapGene.
Watch how to simulate Takara's In-Fusion Cloning with single or multiple fragments in SnapGene.
Watch this series and learn how to simulate single and multi-insert Gibson assembly in SnapGene.
This series shows you how to simulate single and multi insert NEBuilder HiFi Assembly in SnapGene.
Learn how to simulate Golden Gate Assembly, also known as Golden Gate cloning, in SnapGene.

Learn Cloning

Watch this in-depth video series and discover the key concepts of molecular cloning and the plasmid cloning cycle.
Watch this overview of the different molecular cloning methods available today. Discover how they work, their pros and cons and how to choose the best technique for your experiment.
This in-depth course examines the Restriction Enzyme cloning technique, with a detailed overview, pros and cons and top tips for cloning.
Learn more about PCR cloning with this in-depth look, including a detailed overview, pros and cons, top tips for cloning with PCR and a how-to guide for using PCR in SnapGene.
This in-depth course examines the Gateway Cloning technique. It includes a detailed overview, pros and cons, top tips and a how-to guide for using Gateway Cloning in SnapGene.
This in-depth course examines Gibson Assembly, including a detailed overview, pros and cons, top tips and a how-to guide for using Gibson Assembly in SnapGene.
Learn more about In-Fusion cloning with this in-depth look, including a detailed overview, pros and cons, top tips for performing In-Fusion cloning and a how-to guide for the method in SnapGene.
Learn more about Golden Gate assembly with this in-depth look, including a detailed overview, pros and cons and top tips for performing Golden Gate Assembly.
Learn about the Addgene protocols plasmid sequence analysis and annealed oligo cloning in SnapGene.

Cloning Guides

How to improve accuracy, increase efficiency and reduce errors.
Discover the importance of restriction enzymes and the different types available.
An overview of the science behind cloning with restriction enzymes and how to simulate restriction cloning in SnapGene.
Learn the fundamentals of the polymerase chain reaction (PCR).
An overview of PCR cloning, the capture of a PCR product into a vector of interest and how this technique is performed in SnapGene.
Learn how TOPO® cloning is used to clone DNA fragments without the need for restriction enzymes or DNA ligase.
An overview of Thermo Fisher's Gateway Cloning technique and how to simulate the Gateway reaction in SnapGene.
An overview of Gibson Assembly, a technique to create exceptionally large molecular clones from PCR products
Overview of In-Fusion cloning, a remarkably versatile method developed by Takara Biosciences for creating seamless gene fusions.
Learn the steps involved in Golden Gate assembly and how to efficiently plan and simulate your experiment.
Learn the steps involved in CRISPR ranging from designing your gRNA and repair templates to approaches to verify your genome edit.
Discover the most user-friendly molecular biology experience.