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System Requirements
OS Windows 7 or later
macOS 10.10 or later
Fedora Linux 21 or later
Red Hat Linux 7.2 or later
Ubuntu Linux 14.04 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Changes in version 4.2.5 (Sep 19, 2018)

New Functionality

  • Added Accuris DNA Ladders.
    (Requested by Matthias Hamdorf)
  • Added GenDEPOT DNA Ladders.
    (Requested by Joseph Lancman)


  • Added support for batch converting FASTQ files.
    (Requested by Novozymes)
  • Added a check mark in the Enzymes view pull-down menu to indicate when enzymes are sorted by location or distance from the selection.
  • Improved the placement of tooltips for amino acids in translated features.
    (Requested by John Murray)
  • Reduced the minimum allowed primer Tm to 10°C.
    (Requested by Paula Patterson)


  • Ensured that rapid consecutive Return button presses will not cause instability or appearance of multiple copies of a dialog.
    (Reported by Marcy Patrick)
  • Improved the import of primers copied from other programs such as Excel.
    (Reported by Inna Zukher and Nick Martin)
  • Fixed a stability issue when pasting content copied from Microsoft Edge and Internet Explorer.
    (Reported by Novozymes)
  • Improved the horizontal placement of linear map labels when exporting high-DPI images.
    (Reported by Mily Ron)
  • Ensured that the selection is preserved when duplicating lanes amplified by PCR in the Simulate Agarose Gel dialog.
    (Reported by Carles Alvarez)
  • Prevented triggering of the top left hot corner on macOS when switching to Features or Primers view.
    (Reported by Sadik)
  • Improved the opening of SeqBuilder Pro files.
    (Requested by Christopher Skrodzki, Linas Padegimas, and others)
  • Prevented a hang that occurred in some circumstances when simulating an agarose gel by amplifying fragments with manually specified primers.
    (Reported by Ryan Schulze)
  • Fixed an issue with storing ancestors in the history when simulating a procedure.
    (Reported by Nelson Cole)
  • Ensured that the Gateway LR Cloning dialog always recognizes a valid set of templates for recombination.
    (Reported by Georg Huber)
  • Ensured that "Replace Original with Aligned" works as intended, even with unsaved files.
    (Reported by Lauri Lintott)
  • Configured files in a collection to be marked as unsaved after replacing code numbers.
  • Improved the replacement of feature names in collections.
  • Improved stability when saving an alignment after the Description is modified.
  • Fixed an issue with displaying or editing the Description in a collection overview.
  • Improved auto-population of the registration dialog for IP-based licenses.
  • Ensured the reliable display of custom colors in Sequence view.
  • Enabled the opening of text files with macOS traditional line endings.
  • Prevented the Choose Enzymes dialog from being shown twice when using the pull-down menu in Enzymes view.
  • Fixed an issue with using Enzymes → Choose Enzymes from the Browse Common Features dialog.
  • Ensured that the Close button is enabled in various modal dialogs on Windows.
  • Fixed a recent regression with showing run-on translations in Sequence View.
  • Made various other stability improvements.
  • Configured ancestors that are not embedded and thus not cannot be resurrected to be shown in black rather than blue in History view.
  • Improved the display of closely labeled enzymes, features, and primers in linear maps.
  • Improved performance on Windows when saving changes to files.

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