Products

SnapGene® Server

SnapGene format is rapidly becoming the industry standard for sharing plasmid information, and we wanted to make it even easier for companies and organizations to share plasmids online. So we created SnapGene Server.

With SnapGene Server powering your website, visitors can

  • View the beautiful maps you have come to expect from SnapGene
  • Adjust the map format
  • Annotate common features
  • Annotate primer binding sites
  • Obtain a list of features
  • Obtain a list of primers
SnapGene Server is already powering the maps on our own website. Here’s an example.

 DraIII (8626) BspQI - SapI (6465) AbsI - PspXI (6176) AvrII (6154) StuI (6153) SfiI (6107) SexAI * (5921) AgeI (5734) BbvCI (5472) AleI (5246) XbaI (5176) KpnI (5167) Acc65I (5163) AscI - BssHII (4955) EcoRI (4822) AarI (4260) BsrGI (276) SpeI (332) CMV enhancer SnaBI (673) ATG BspDI - ClaI (971) SbfI (1247) Bsu36I (2266) BmgBI (3510) PmlI (3789) FspAI (3950) NruI (4240) 3xFLAG-dCas9/pCMV-7.1 8848 bp
DraIII  (8626)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BspQI  (6465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (6465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
AbsI  (6176)
1 site
C C T C G A G G G G A G C T C C
PspXI  (6176)
1 site
V C T C G A G B B G A G C T C V
AvrII  (6154)
1 site
C C T A G G G G A T C C
StuI  (6153)
1 site
A G G C C T T C C G G A
SfiI  (6107)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI  (5921)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AgeI  (5734)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BbvCI  (5472)
1 site
C C T C A G C G G A G T C G
AleI  (5246)
1 site
C A C N N N N G T G G T G N N N N C A C
XbaI  (5176)
1 site
T C T A G A A G A T C T
KpnI  (5167)
1 site
G G T A C C C C A T G G
Acc65I  (5163)
1 site
G G T A C C C C A T G G
AscI  (4955)
1 site
G G C G C G C C C C G C G C G G
BssHII  (4955)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoRI  (4822)
1 site
G A A T T C C T T A A G
AarI  (4260)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BsrGI  (276)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (332)
1 site
A C T A G T T G A T C A
SnaBI  (673)
1 site
T A C G T A A T G C A T
BspDI  (971)
1 site
A T C G A T T A G C T A
ClaI  (971)
1 site
A T C G A T T A G C T A
SbfI  (1247)
1 site
C C T G C A G G G G A C G T C C
Bsu36I  (2266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BmgBI  (3510)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
PmlI  (3789)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
FspAI  (3950)
1 site
R T G C G C A Y Y A C G C G T R
NruI  (4240)
1 site
T C G C G A A G C G C T
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 1:  
   1000 .. 1026  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 2:  
   1027 .. 1029  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 3:  
   1030 .. 3516  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 4:  
   3517 .. 3519  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 5:  
   3520 .. 5103  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7401 .. 8192  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   8193 .. 8261  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
hGH poly(A) signal
5189 .. 5811  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5189 .. 5811  =  623 bp
human growth hormone polyadenylation signal
ori
6642 .. 7230  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6642 .. 7230  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
8393 .. 8848  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
8393 .. 8848  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
318 .. 697  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
318 .. 697  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5840 .. 6169  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5840 .. 6169  =  330 bp
SV40 enhancer and early promoter
CMV promoter
698 .. 901  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
698 .. 901  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
AmpR promoter
8262 .. 8366  =  105 bp
AmpR promoter
8262 .. 8366  =  105 bp
3xFLAG
931 .. 996  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
3xFLAG
931 .. 996  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
SV40 NLS
5116 .. 5136  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5116 .. 5136  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
ATG
928 .. 930  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
928 .. 930  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
6020 .. 6155  =  136 bp
SV40 origin of replication
SV40 ori
6020 .. 6155  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

You can do the same, or use JavaScript to customize in various ways.

Visitors Can Even Create Their Own Maps

Visitors to your website can upload sequences, and SnapGene Server will automatically generate interactive maps.

Import and Export Common Formats

No matter what program scientists use to plan experiments, they should be able to see their data in an easy-to-read map, so we've taken care that you can import* and export** common file formats.

* ApE, CLC Bio, Clone Manager, DNAssist, DNADynamo, DNASTAR Lasergene®, DNA Strider, DS Gene, EMBL (ENA), EnzymeX, GenBank / DDBJ, Gene Construction Kit®, GeneTool, Jellyfish, MacVector, pDRAW32, Serial Cloner, Vector NTI®, Visual Cloning

** FASTA, GenBank, EMBL

Want to power your plasmid maps with SnapGene Server?

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