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Gateway® Cloning

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System Requirements
OS Windows 10, 8, 7, Vista or XP
Mac OS X 10.7 or later
Ubuntu Linux 14.04 or later
Fedora Linux 21 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Plasmids can be constructed without restriction enzymes using Gateway® cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

For standard Gateway® cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway® cloning allows up to four fragments to be inserted simultaneously.

SnapGene simplifies Gateway® cloning by automating the primer design. To plan a Gateway® cloning procedure, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

Please click below to see screenshots and a brief tutorial video. A list of annotated Gateway® cloning vectors is available online, and is embedded in the SnapGene software.

attB Insert

Gateway Cloning Tutorial Video

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