Gateway® Cloning

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System Requirements
OS Windows 7 or later
macOS 10.10 or later
Fedora Linux 21 or later
Red Hat Linux 7.2 or later
Ubuntu Linux 14.04 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Plasmids can be constructed without restriction enzymes using Gateway® cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

For standard Gateway® cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway® cloning allows up to four fragments to be inserted simultaneously.

SnapGene simplifies Gateway® cloning by automating the primer design. To plan a Gateway® cloning procedure, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

Please click below to see screenshots and a brief tutorial video. A list of annotated Gateway® cloning vectors is available online, and is embedded in the SnapGene software.

attB Insert

Gateway Cloning Tutorial Video

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