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Gibson Assembly®

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System Requirements
OS Windows 10, 8, 7, Vista or XP
Mac OS X 10.7 or later
Ubuntu Linux 14.04 or later
Fedora Linux 21 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Many researchers are turning to Gibson Assembly to insert fragments into a plasmid without the use of restriction enzymes. SnapGene simulates this method with an intuitive interface.

For Gibson Assembly, the DNA segments to be joined are amplified by PCR to create overlapping ends. Then the amplified products are incubated with assembly enzymes, and the mixture is transformed into bacteria.

SnapGene simplifies Gibson Assembly by automating the primer design. To plan a Gibson Assembly reaction, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

Please click below to see screenshots and a brief tutorial video.

Vector

Gibson Assembly Tutorial Video

These sites provide further information about Gibson Assembly:

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