In-Fusion® Cloning

Clontech Recommends SnapGene
SnapGene simulates In-Fusion cloning with elegant simplicity. The research scientists at Clontech use SnapGene, and I am pleased to recommend this software as the ideal partner for our In-Fusion cloning kits.
Andrew Farmer, D.Phil.
VP of Research and Development
Clontech Laboratories Inc.
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System Requirements
OS Windows 10, 8, 7, Vista or XP
Mac OS X 10.7 or later
Ubuntu Linux 14.04 or later
Fedora Linux 21 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Clontech's In-Fusion cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene is the first software to simulate this procedure.

For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends.

SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers.

Please click below to see screenshots, a brief tutorial video, and In-Fusion-ready vectors from Clontech.


In-Fusion Cloning Tutorial Video

Download annotated files for In-Fusion-ready linearized vectors from Clontech:

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