Resources

In-Fusion® Cloning

Clontech Recommends SnapGene
SnapGene simulates In-Fusion cloning with elegant simplicity. The research scientists at Clontech use SnapGene, and I am pleased to recommend this software as the ideal partner for our In-Fusion cloning kits.
Andrew Farmer, D.Phil.
VP of Research and Development
Clontech Laboratories Inc.
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System Requirements
OS Windows 7 or later
macOS 10.10 or later
Fedora Linux 21 or later
Red Hat Linux 7.2 or later
Ubuntu Linux 14.04 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

Clontech's In-Fusion cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene is the first software to simulate this procedure.

For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends.

SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers.

Please click below to see screenshots, a brief tutorial video, and In-Fusion-ready vectors from Clontech.

Vector

In-Fusion Cloning Tutorial Video

Download annotated files for In-Fusion-ready linearized vectors from Clontech:

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