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Plasmid Files

pEZSeq™-Amp

Blue/white screening vector with an ampicillin resistance marker for high efficiency blunt end cloning.

To see this sequence with restriction sites, features, and translations, please download
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pEZSeq-Amp.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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Z-Rev (61 .. 84) AlwNI - CaiI (1737) GsaI - PspFI (1629) BseYI (1625) BciVI - BfuI (1524) BanIII - Bsa29I - BseCI - BshVI - BspDI - BspXI - Bsu15I - BsuTUI - ClaI (1421) ApaI - BanII - Eco24I - EcoT38I - FriOI (1327) Bsp120I - DraII - EcoO109I - PspOMI (1323) AhdI - AspEI - BmeRI - DriI - Eam1105I (1246) BsaI - Bso31I - BspTNI - Eco31I (1180) Bse118I - BsrFI - BssAI - Cfr10I (1161) NmeAIII (1099) AseI - PshBI - VspI (1071) AccBSI - BsrBI - MbiI (62) HindIII (109) SalI (115) AccI - FblI - XmiI (116) HincII - HindII (117) EcoRI (121) Z-For (147 .. 170) PvuII (213) Bst6I - Eam1104I - EarI - Ksp632I (222) SmiI - SwaI (296) BsaAI - BstBAI - Ppu21I (365) Bpu10I (445) Asp700I - MroXI - PdmI - XmnI (646) AcyI - BsaHI - BssNI - BstACI - Hin1I - Hsp92I (706) TatI (763) Acc113I - BmcAI - ScaI - ZrmI (765) TsoI (848) XcmI (863) pEZSeq™-Amp 2056 bp
AlwNI  (1737)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
CaiI  (1737)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different CaiI sites may not be compatible.
GsaI  (1629)
1 site
C C C A G C G G G T C G
PspFI  (1629)
1 site
C C C A G C G G G T C G
BseYI  (1625)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BciVI  (1524)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BfuI  (1524)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BfuI may be hard to ligate.
Sticky ends from different BfuI sites may not be compatible.
BanIII  (1421)
1 site
A T C G A T T A G C T A
Bsa29I  (1421)
1 site
A T C G A T T A G C T A
BseCI  (1421)
1 site
A T C G A T T A G C T A
BshVI  (1421)
1 site
A T C G A T T A G C T A
BspDI  (1421)
1 site
A T C G A T T A G C T A
BspXI  (1421)
1 site
A T C G A T T A G C T A
Bsu15I  (1421)
1 site
A T C G A T T A G C T A
BsuTUI  (1421)
1 site
A T C G A T T A G C T A
ClaI  (1421)
1 site
A T C G A T T A G C T A
ApaI  (1327)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (1327)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Eco24I  (1327)
1 site
G R G C Y C C Y C G R G

Sticky ends from different Eco24I sites may not be compatible.
EcoT38I  (1327)
1 site
G R G C Y C C Y C G R G

Sticky ends from different EcoT38I sites may not be compatible.
FriOI  (1327)
1 site
G R G C Y C C Y C G R G

Sticky ends from different FriOI sites may not be compatible.
Bsp120I  (1323)
1 site
G G G C C C C C C G G G
DraII  (1323)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different DraII sites may not be compatible.
EcoO109I  (1323)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (1323)
1 site
G G G C C C C C C G G G
AhdI  (1246)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AspEI  (1246)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AspEI may be hard to ligate.
Sticky ends from different AspEI sites may not be compatible.
BmeRI  (1246)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by BmeRI may be hard to ligate.
Sticky ends from different BmeRI sites may not be compatible.
DriI  (1246)
1 site