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Plasmid Files

pBS-Hsp70-Cas9

Bacterial plasmid encoding S. pyogenes Cas9 under control of the Drosophila hsp70 promoter and poly(A) signal.

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pBS-Hsp70-Cas9 Sequence and MappBS-Hsp70-Cas9.dna
Map and Sequence File   
Sequence Author:  O'Connor-Giles Lab / Addgene #46294
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 NotI (6411) NotI (670) ATG SV40 NLS pBS-Hsp70-Cas9 8699 bp
NotI  (6411)
2 sites
G C G G C C G C C G C C G G C G
NotI  (670)
2 sites
G C G G C C G C C G C C G G C G
Cas9
1280 .. 5380  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1280 .. 5380  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
hsp70 poly(A)
5508 .. 6380  =  873 bp
polyadenlyation signal from the Drosophila
melanogaster
hsp70Ab gene
hsp70 poly(A)
5508 .. 6380  =  873 bp
polyadenlyation signal from the Drosophila
melanogaster
hsp70Ab gene
AmpR
7714 .. 8574  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7714 .. 8505  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7714 .. 8574  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   8506 .. 8574  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
7714 .. 8574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
6955 .. 7543  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6955 .. 7543  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
6 .. 461  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
6 .. 461  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
hsp70 promoter
692 .. 1144  =  453 bp
Drosophila melanogaster hsp70Bb promoter
hsp70 promoter
692 .. 1144  =  453 bp
Drosophila melanogaster hsp70Bb promoter
AmpR promoter
8575 .. 8679  =  105 bp
AmpR promoter
8575 .. 8679  =  105 bp
3xFLAG
1163 .. 1228  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
3xFLAG
1163 .. 1228  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags,
followed by an enterokinase cleavage site
nucleoplasmin NLS
5381 .. 5428  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from
nucleoplasmin
nucleoplasmin NLS
5381 .. 5428  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from
nucleoplasmin
SV40 NLS
1235 .. 1255  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
1235 .. 1255  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
ATG
1160 .. 1162  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1160 .. 1162  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
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