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Plasmid Files

pCLIP-Cas9-Nickase-TRE3G-ZsGreen

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and the green fluorescent protein ZsGreen expressed from a Tet-responsive promoter.

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pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,775) BpvUI - Ple19I - PvuI (10,341) Acc16I - AviII - FspI - NsbI (10,193) AasI - DrdI - DseDI (9189) AflIII - BspLU11I - PciI - PscI (9081) SmaI (9061) Cfr9I - TspMI - XmaI - XmaCI (9059) AspA2I - AvrII - BlnI - XmaJI (9038) CsiI - MabI - SexAI * (8805) MssI - PmeI (8713) AanI - PsiI (8663) KpnI (8328) Acc65I - Asp718I (8324) AxyI - Bse21I - Bsu36I - Eco81I (8320) Cfr42I - KspI - SacII - Sfr303I - SgrBI - SstII (8105) EcoRI (7578) Bsp1407I - BsrGI - BstAUI - SspBI (7432) AspI - PflFI - PsyI - Tth111I (7116) SgrAI (6969) BamHI (6816) BsaBI * - Bse8I - BseJI * - MamI * (6138) AhlI - BcuI - SpeI (41) FauNDI - NdeI (276) BstSNI - Eco105I - SnaBI (382) AsuNHI - NheI (405) BmtI - BspOI (409) BsePI - BssHII - PauI - PteI (745) Bsp68I - BtuMI - NruI * - RruI (867) BspQI - LguI - PciSI - SapI (1136) FseI - RigI (1184) MfeI - MunI (1223) BbvCI (1458) AleI - OliI (1611) AbsI - PspXI (2238) XbaI (2623) AfeI - Aor51HI - Eco47III (2630) Bsp120I - PspOMI (3995) ApaI (3999) PasI (4409) AdeI - DraIII (5039) AcvI - BbrPI - Eco72I - PmaCI - PmlI - PspCI (5429)