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Plasmid Files

pCLIP-Cas9-Nickase-TRE3G-ZsGreen

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and the green fluorescent protein ZsGreen expressed from a Tet-responsive promoter.

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pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,775) PvuI (10,341) FspI (10,193) DrdI (9189) AflIII - PciI (9081) SmaI (9061) TspMI - XmaI (9059) AvrII (9038) SexAI * (8805) PmeI (8713) PsiI (8663) KpnI (8328) Acc65I (8324) Bsu36I (8320) SacII (8105) EcoRI (7578) BsrGI (7432) PflFI - Tth111I (7116) SgrAI (6969) BamHI (6816) BsaBI * (6138) PmlI (5429) SpeI (41) NdeI (276) SnaBI (382) NheI (405) BmtI (409) BssHII (745) NruI * (867) BspQI - SapI (1136) FseI (1184) MfeI (1223) BbvCI (1458) AleI (1611) AbsI - PspXI (2238) XbaI (2623) AfeI (2630) PspOMI (3995) ApaI (3999) PasI (4409) DraIII (5039) pCLIP-Cas9-Nick