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Plasmid Files

pCLIP-Cas9-Nickase-TRE3G-tRFP

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and the red fluorescent protein TurboRFP expressed from a Tet-responsive promoter.

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pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,775) PvuI (10,341) FspI (10,193) DrdI (9189) AflIII - PciI (9081) SmaI (9061) TspMI - XmaI (9059) AvrII (9038) SexAI * (8805) PmeI (8713) PsiI (8663) KpnI (8328) Acc65I (8324) SalI (8200) SacII (8105) EcoRI (7578) PshAI (7509) SfiI (7345) BsrGI (6919) BamHI (6816) BsaBI * (6138) SpeI (41) NdeI (276) SnaBI (382) NheI (405) BmtI (409) BssHII (745) NruI * (867) BspQI - SapI (1136) FseI (1184) MfeI (1223) BbvCI (1458) AleI (1611) AbsI - PspXI (2238) XbaI (2623) AfeI (2630) PspOMI (3995) ApaI (3999) PmlI (5429) pCLIP-Cas9-Nickase-TRE3G-tRFP 10,893 bp