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pCLIP-Cas9-Nickase-hCMV-Puro

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and a puromycin resistance marker expressed from the human CMV promoter.

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pCLIP-Cas9(D10a)-Nickase-hCMV-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,941) PvuI (10,507) FspI (10,359) DrdI (9355) AflIII - PciI (9247) SmaI (9227) TspMI - XmaI (9225) AvrII (9204) PmeI (8879) PsiI (8829) KpnI (8494) Acc65I (8490) Bsu36I (8486) SalI (8366) BstEII (7278) RsrII (7260) BsiWI (7200) PflFI - Tth111I (7186) BsaBI * (6403) PmlI (5694) NheI (405) BmtI (409) NruI * (867) BspQI - SapI (1136) MfeI (1219) BbvCI (1454) AleI (1607) BsmBI (2766) PspXI (2882) XbaI (2888) AfeI (2895) PspOMI (4260) ApaI (4264) pCLIP-Cas9-Nickase-hCMV-Puro 11,059 bp
SspI  (10,941)
1 site
A A T A T T T T A T A A
PvuI  (10,507)
1 site
C G A T C G G C T A G C
FspI  (10,359)
1 site
T G C G C A A C G C G T
DrdI  (9355)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AflIII  (9247)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (9247)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SmaI  (9227)
1 site
C C C G G G