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pDCC6

Drosophila CRISPR/Cas9 genome editing vector for expressing a single guide RNA (sgRNA) together with Cas9.

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pDCC6.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Duchek Lab / Addgene #59985
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BbsI (479) BbsI (501) pDCC6 8154 bp
BbsI  (479)
2 sites		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (501)
2 sites		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Cas9
1225 .. 5325  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1225 .. 5325  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6353 .. 6421  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6422 .. 7213  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6353 .. 7213  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7384 .. 7972  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7384 .. 7972  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
dU6 promoter
84 .. 483  =  400 bp
RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987)
dU6 promoter
84 .. 483  =  400 bp
RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987)
hsp70 promoter
663 .. 1004  =  342 bp
Drosophila melanogaster hsp70Bb promoter
hsp70 promoter
663 .. 1004  =  342 bp
Drosophila melanogaster hsp70Bb promoter
SV40 poly(A) signal
6122 .. 6256  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
6122 .. 6256  =  135 bp
SV40 polyadenylation signal
gRNA scaffold
502 .. 577  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
502 .. 577  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
3xFLAG
1108 .. 1173  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
1108 .. 1173  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
1180 .. 1200  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
1180 .. 1200  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5329 .. 5349  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5329 .. 5349  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
polIII terminator
578 .. 583  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
578 .. 583  =  6 bp
RNA polymerase III transcription terminator
ATG
1105 .. 1107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1105 .. 1107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ORF:  1105 .. 5361  =  4257 bp
ORF:  1418 amino acids  =  164.2 kDa
ORF:  5626 .. 6099  =  474 bp
ORF:  157 amino acids  =  18.1 kDa
ORF:  6353 .. 7213  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  799 .. 3741  =  2943 bp
ORF:  980 amino acids  =  104.1 kDa
ORF:  6817 .. 7083  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2571 .. 2864  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  3135 .. 3584  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
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