Plasmid Files

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BbsI  (479) 2 sites

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

BbsI  (501) 2 sites

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

Cas9 1225 .. 5325  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

Cas9 1225 .. 5325  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

AmpR 6353 .. 7213  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      6353 .. 6421  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6353 .. 7213  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      6422 .. 7213  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6353 .. 7213  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

ori 7384 .. 7972  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 7384 .. 7972  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

dU6 promoter 84 .. 483  =  400 bp RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987)

dU6 promoter 84 .. 483  =  400 bp RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987)

hsp70 promoter 663 .. 1004  =  342 bp Drosophila melanogaster hsp70Bb promoter

hsp70 promoter 663 .. 1004  =  342 bp Drosophila melanogaster hsp70Bb promoter

SV40 poly(A) signal 6122 .. 6256  =  135 bp SV40 polyadenylation signal

SV40 poly(A) signal 6122 .. 6256  =  135 bp SV40 polyadenylation signal

gRNA scaffold 502 .. 577  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

gRNA scaffold 502 .. 577  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

3xFLAG 1108 .. 1173  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

3xFLAG 1108 .. 1173  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

SV40 NLS 1180 .. 1200  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 1180 .. 1200  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 5329 .. 5349  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 5329 .. 5349  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

polIII terminator 578 .. 583  =  6 bp RNA polymerase III transcription terminator

polIII terminator 578 .. 583  =  6 bp RNA polymerase III transcription terminator

ATG 1105 .. 1107  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 1105 .. 1107  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

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