Plasmid Files |
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![]() | pDCC6.dna |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
Cas9 1225 .. 5325 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
Cas9 1225 .. 5325 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes MGAS15252 Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
AmpR 6353 .. 7213 = 861 bp 286 amino acids = 31.6 kDa Segment 1: signal sequence 6353 .. 6421 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6353 .. 7213 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 6422 .. 7213 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6353 .. 7213 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 7384 .. 7972 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 7384 .. 7972 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
dU6 promoter 84 .. 483 = 400 bp RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987) |
dU6 promoter 84 .. 483 = 400 bp RNA polymerase III promoter for Drosophila U6-2 snRNA (Das et al., 1987) |
hsp70 promoter 663 .. 1004 = 342 bp Drosophila melanogaster hsp70Bb promoter |
hsp70 promoter 663 .. 1004 = 342 bp Drosophila melanogaster hsp70Bb promoter |
SV40 poly(A) signal 6122 .. 6256 = 135 bp SV40 polyadenylation signal |
SV40 poly(A) signal 6122 .. 6256 = 135 bp SV40 polyadenylation signal |
gRNA scaffold 502 .. 577 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
gRNA scaffold 502 .. 577 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
3xFLAG 1108 .. 1173 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
3xFLAG 1108 .. 1173 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
SV40 NLS 1180 .. 1200 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 1180 .. 1200 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 5329 .. 5349 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 5329 .. 5349 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
polIII terminator 578 .. 583 = 6 bp RNA polymerase III transcription terminator |
polIII terminator 578 .. 583 = 6 bp RNA polymerase III transcription terminator |
ATG 1105 .. 1107 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 1105 .. 1107 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ORF: 1105 .. 5361 = 4257 bp ORF: 1418 amino acids = 164.2 kDa |
ORF: 5626 .. 6099 = 474 bp ORF: 157 amino acids = 18.1 kDa |
ORF: 6353 .. 7213 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 799 .. 3741 = 2943 bp ORF: 980 amino acids = 104.1 kDa |
ORF: 6817 .. 7083 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 2571 .. 2864 = 294 bp ORF: 97 amino acids = 10.8 kDa |
ORF: 3135 .. 3584 = 450 bp ORF: 149 amino acids = 17.3 kDa |
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