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Plasmid Files

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide.

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Vale Lab / Addgene #60910
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SbfI (14,668) SalI (14,658) PacI (14,637) Bsu36I (14,192) RsrII (12,052) BamHI (12,043) SmaI (7797) TspMI - XmaI (7795) SacII (7791) MluI (7758) NheI (340) BmtI (344) SgrAI (382) AfeI (468) PvuI (1432) FspI (1578) SfiI (3146) AgeI (3683) HpaI (4981) BbvCI (6703) AleI (6856) EcoRI (7501)