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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide.

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE Sequence and MappHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE.dna
Map and Sequence File   
Sequence Author:  Vale Lab / Addgene #60910
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 SbfI (14,668) SalI (14,658) PacI (14,637) Bsu36I (14,192) SV40 NLS RsrII (12,052) BamHI (12,043) SV40 NLS SV40 NLS SmaI (7797) TspMI - XmaI (7795) SacII (7791) MluI (7758) SV40 NLS ATG NheI (340) BmtI (344) SgrAI (382) AfeI (468) PvuI (1432) FspI (1578) SfiI (3146) AgeI (3683) HpaI (4981) BbvCI (6703) AleI (6856) EcoRI (7501) pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 14,911 bp
SbfI  (14,668)
1 site
C C T G C A G G G G A C G T C C
SalI  (14,658)
1 site
G T C G A C C A G C T G
PacI  (14,637)
1 site
T T A A T T A A A A T T A A T T
Bsu36I  (14,192)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
RsrII  (12,052)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BamHI  (12,043)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (7797)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (7795)
1 site
C C C G G G G G G C C C
XmaI  (7795)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SacII  (7791)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
MluI  (7758)
1 site
A C G C G T T G C G C A
NheI  (340)
1 site
G C T A G C C G A T C G
BmtI  (344)
1 site
G C T A G C C G A T C G
SgrAI  (382)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AfeI  (468)
1 site
A G C G C T T C G C G A
PvuI  (1432)
1 site
C G A T C G G C T A G C
FspI  (1578)
1 site
T G C G C A A C G C G T
SfiI  (3146)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AgeI  (3683)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
HpaI  (4981)
1 site
G T T A A C C A A T T G
BbvCI  (6703)
1 site
C C T C A G C G G A G T C G
AleI  (6856)
1 site
C A C N N N N G T G G T G N N N N C A C
EcoRI  (7501)
1 site
G A A T T C C T T A A G
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 1:  
   7810 .. 7836  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 2:  
   7837 .. 7839  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 3:  
   7840 .. 10,326  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 4:  
   10,327 .. 10,329  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
   Segment 5:  
   10,330 .. 11,913  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in
the RuvC catalytic domain and the H840A mutation
in the HNH catalytic domain
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 1:  GCN4_v4  
   12,088 .. 12,144  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 2:  
   12,145 .. 12,159  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 3:  GCN4_v4  
   12,160 .. 12,216  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 4:  
   12,217 .. 12,231  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 5:  GCN4_v4  
   12,232 .. 12,288  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 6:  
   12,289 .. 12,303  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 7:  GCN4_v4  
   12,304 .. 12,360  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 8:  
   12,361 .. 12,375  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 9:  GCN4_v4  
   12,376 .. 12,432  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 10:  
   12,433 .. 12,447  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 11:  GCN4_v4  
   12,448 .. 12,504  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 12:  
   12,505 .. 12,519  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 13:  GCN4_v4  
   12,520 .. 12,576  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 14:  
   12,577 .. 12,591  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 15:  GCN4_v4  
   12,592 .. 12,648  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 16:  
   12,649 .. 12,663  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 17:  GCN4_v4  
   12,664 .. 12,720  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 18:  
   12,721 .. 12,735  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 19:  GCN4_v4  
   12,736 .. 12,792  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 20:  
   12,793 .. 12,807  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 21:  GCN4_v4  
   12,808 .. 12,864  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 22:  
   12,865 .. 12,879  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 23:  GCN4_v4  
   12,880 .. 12,936  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 24:  
   12,937 .. 12,951  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 25:  GCN4_v4  
   12,952 .. 13,008  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 26:  
   13,009 .. 13,023  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 27:  GCN4_v4  
   13,024 .. 13,080  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 28:  
   13,081 .. 13,095  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 29:  GCN4_v4  
   13,096 .. 13,152  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 30:  
   13,153 .. 13,167  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 31:  GCN4_v4  
   13,168 .. 13,224  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 32:  
   13,225 .. 13,239  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 33:  GCN4_v4  
   13,240 .. 13,296  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 34:  
   13,297 .. 13,311  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 35:  GCN4_v4  
   13,312 .. 13,368  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 36:  
   13,369 .. 13,383  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 37:  GCN4_v4  
   13,384 .. 13,440  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 38:  
   13,441 .. 13,455  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 39:  GCN4_v4  
   13,456 .. 13,512  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 40:  
   13,513 .. 13,527  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 41:  GCN4_v4  
   13,528 .. 13,584  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 42:  
   13,585 .. 13,599  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 43:  GCN4_v4  
   13,600 .. 13,656  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 44:  
   13,657 .. 13,671  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 45:  GCN4_v4  
   13,672 .. 13,728  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 46:  
   13,729 .. 13,743  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
   Segment 47:  GCN4_v4  
   13,744 .. 13,800  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
Product: 24 tandem copies of the GCN4 peptide
optimized to improve solubility while preserving
binding to an scFv-GFP fusion protein (Tanenbaum
et al., 2014)
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1014 .. 1082  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1083 .. 1874  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from
HIV-1
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from
HIV-1
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
AmpR promoter
909 .. 1013  =  105 bp
AmpR promoter
909 .. 1013  =  105 bp
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1
polyprotein
Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides.
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1
polyprotein
Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides.
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin)
epitope tag
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin)
epitope tag
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
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