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Plasmid Files

DsRed.T3

Rapidly maturing tetrameric variant of DsRed fluorescent protein.

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DsRed.T3 Sequence and MapDsRed.T3.dna
Map and Sequence File   
Sequence Author:  Glick Lab
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 600 400 200 End (678) AleI (628) BsgI (580) MspA1I - PvuII (564) MscI (547) BstXI (543) PflMI * (519) SexAI * (518) EcoO109I (489) BstYI (479) HaeII (447) PspFI (429) BseYI (425) BbsI (421) MmeI (416) PstI - SbfI (341) BmeT110I (263) AvaI - BsoBI (262) BmrI (183) AhdI (180) TaqII (136) BaeGI - Bme1580I (121) BanI (118) BssS α I (73) FspI - FspAI (49) AatII (20) ZraI (18) BsaHI (17) Start (0) DsRed.T3 678 bp
End  (678)
0 sites
AleI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
BsgI  (580)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MspA1I  (564)
1 site
C M G C K G G K C G M C
PvuII  (564)
1 site
C A G C T G G T C G A C
MscI  (547)
1 site
T G G C C A A C C G G T
BstXI  (543)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PflMI  (519)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
SexAI  (518)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
EcoO109I  (489)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BstYI  (479)
1 site
R G A T C Y Y C T A G R
HaeII  (447)
1 site
R G C G C Y Y C G C G R
PspFI  (429)
1 site
C C C A G C G G G T C G
BseYI  (425)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
MmeI  (416)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (341)
1 site
C T G C A G G A C G T C
SbfI  (341)
1 site
C C T G C A G G G G A C G T C C
BmeT110I  (263)
1 site
C Y C G R G G R G C Y C
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmrI  (183)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
TaqII  (136)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BaeGI  (121)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (121)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BanI  (118)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BssSαI  (73)
1 site
C A C G A G G T G C T C
FspI  (49)
1 site
T G C G C A A C G C G T
FspAI  (49)
1 site
R T G C G C A Y Y A C G C G T R
AatII  (20)
1 site
G A C G T C C T G C A G
ZraI  (18)
1 site
G A C G T C C T G C A G
BsaHI  (17)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
Start  (0)
0 sites
DsRed.T3
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: rapidly maturing tetrameric variant of
DsRed fluorescent protein (Bevis and Glick, 2002)
mammalian codon-optimized
DsRed.T3
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: rapidly maturing tetrameric variant of
DsRed fluorescent protein (Bevis and Glick, 2002)
mammalian codon-optimized
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