Resources
Plasmid Files

EYFP

Enhanced yellow fluorescent protein.

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EYFP Sequence and MapEYFP.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 EYFP End (720) BsrGI (710) BstYI (500) PfoI * (281) Bts α I (214) PstI (209) SfcI (205) BssS α I (181) BsrFI (151) BtgZI (122) BanI (36) BseRI (31) Start (0) EYFP 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
PstI  (209)
1 site
C T G C A G G A C G T C
SfcI  (205)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
EYFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: enhanced YFP
mammalian codon-optimized
EYFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: enhanced YFP
mammalian codon-optimized
EYFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.8 kDa
Product: enhanced YFP
mammalian codon-optimized
EYFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
Product: enhanced YFP
mammalian codon-optimized
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