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Plasmid Files

Kaede

Kaede green-to-red photoswitchable fluorescent protein.

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Kaede Sequence and MapKaede.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (681) PflFI - Tth111I (670) BsiHKAI (641) Bts α I (582) AcuI - Eco57MI (504) HincII (461) EcoP15I (456) BbsI (412) BtgZI (291) BsmI (181) DrdI (130) BaeGI - Bme1580I * (65) BanI (62) BsrDI (57) Start (0) Kaede 681 bp
End  (681)
0 sites
PflFI  (670)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (670)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiHKAI  (641)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BtsαI  (582)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
AcuI  (504)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (504)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
HincII  (461)
1 site
G T Y R A C C A R Y T G
EcoP15I  (456)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgZI  (291)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
BanI  (62)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsrDI  (57)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
Start  (0)
0 sites
Kaede
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: Kaede green-to-red photoconvertible
fluorescent protein (Ando et al., 2002)
Kaede
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: Kaede green-to-red photoconvertible
fluorescent protein (Ando et al., 2002)
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