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Plasmid Files

hmAzami-Green

Humanized monomeric Azami-Green fluorescent protein.

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hmAzami-Green Sequence and MaphmAzami-Green.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 hmAzami-Green End (681) MscI * (646) BtgI (641) BpmI (511) SfcI (507) BmgBI (471) BsaAI (444) BanII (425) BbsI (412) MslI (325) BseRI - BsgI (288) BmrI (276) EcoO109I (200) HaeII - PluTI (146) SfoI (144) NarI (143) KasI (142) AlwNI * - PflMI * (120) BclI * (11) Start (0) hmAzami-Green 681 bp
End  (681)
0 sites
MscI  (646)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
BtgI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BpmI  (511)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
SfcI  (507)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BmgBI  (471)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BsaAI  (444)
1 site
Y A C G T R R T G C A Y
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BseRI  (288)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BsgI  (288)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BmrI  (276)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
EcoO109I  (200)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
HaeII  (146)
1 site
R G C G C Y Y C G C G R
PluTI  (146)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (144)
1 site
G G C G C C C C G C G G
NarI  (143)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (142)
1 site
G G C G C C C C G C G G
AlwNI  (120)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
PflMI  (120)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hmAzami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
Product: humanized monomeric Azami-Green
fluorescent protein
mammalian codon-optimized
hmAzami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
Product: humanized monomeric Azami-Green
fluorescent protein
mammalian codon-optimized
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