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Plasmid Files

hmAzami-Green

Humanized monomeric Azami-Green fluorescent protein.

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hmAzami-Green.dna
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Sequence Author:  MBL International
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600 400 200 hmAzami-Green End (681) BalI - MlsI * - MluNI - MscI * - Msp20I (646) BstDSI - BtgI (641) BpmI - GsuI (511) BfmI - BstSFI - SfcI (507) AjiI - BmgBI - BtrI (471) BsaAI - BstBAI - Ppu21I (444) BanII - Eco24I - EcoT38I - FriOI (425) BbsI - BpiI - BpuAI - BstV2I (412) MslI - RseI - SmiMI (325) BseRI - BsgI (288) BfiI - BmrI - BmuI (276) DraII - EcoO109I (200) BbeI - BfoI - BstH2I - HaeII - PluTI (146) DinI - EgeI - EheI - SfoI (144) Mly113I - NarI (143) KasI - SspDI (142) AccB7I * - AlwNI * - CaiI * - PflMI * - Van91I * (120) BclI * - FbaI * - Ksp22I * (11) Start (0) hmAzami-Green 681 bp
End  (681)
0 sites
BalI  (646)
1 site
T G G C C A A C C G G T
MlsI  (646)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
MluNI  (646)
1 site
T G G C C A A C C G G T
MscI  (646)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
Msp20I  (646)
1 site
T G G C C A A C C G G T
BstDSI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BstDSI sites may not be compatible.
BtgI  (641)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BpmI  (511)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
GsuI  (511)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Sticky ends from different GsuI sites may not be compatible.
After cleavage, GsuI can remain bound to DNA and alter its electrophoretic mobility.
GsuI is typically used at 30°C, but is 70% active at 37°C.
For full activity, add fresh S-adenosylmethionine (SAM).
BfmI  (507)
1 site
C T R Y A G G A Y R T C

Sticky ends from different BfmI sites may not be compatible.
BstSFI  (507)
1 site
C T R Y A G G A Y R T C

Sticky ends from different BstSFI sites may not be compatible.
SfcI  (507)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AjiI  (471)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by AjiI will not always regenerate an AjiI site.
BmgBI  (471)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BtrI  (471)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BtrI will not always regenerate a BtrI site.
BsaAI  (444)
1 site
Y A C G T R R T G C A Y
BstBAI  (444)
1 site
Y A C G T R R T G C A Y
Ppu21I  (444)
1 site
Y A C G T R R T G C A Y
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Eco24I  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different Eco24I sites may not be compatible.
EcoT38I  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different EcoT38I sites may not be compatible.
FriOI  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different FriOI sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BpuAI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpuAI sites may not be compatible.
BstV2I  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BstV2I sites may not be compatible.
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
RseI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SmiMI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BseRI  (288)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (288)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BfiI  (276)
1 site
A C T G G G ( N ) 4 ( N ) 1-3 T G A C C C ( N ) 4

Efficient cleavage requires at least two copies of the BfiI recognition sequence.
Sticky ends from different BfiI sites may not be compatible.
Unlike most restriction enzymes, BfiI can cleave DNA in the absence of magnesium.
BmrI  (276)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BmuI  (276)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmuI may be hard to ligate.
Sticky ends from different BmuI sites may not be compatible.
DraII  (200)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different DraII sites may not be compatible.
EcoO109I  (200)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BbeI  (146)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the BbeI recognition sequence.
BfoI  (146)
1 site
R G C G C Y Y C G C G R
BstH2I  (146)
1 site
R G C G C Y Y C G C G R
HaeII  (146)
1 site
R G C G C Y Y C G C G R
PluTI  (146)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
DinI  (144)
1 site
G G C G C C C C G C G G
EgeI  (144)
1 site
G G C G C C C C G C G G
EheI  (144)
1 site
G G C G C C C C G C G G
SfoI  (144)
1 site
G G C G C C C C G C G G
Mly113I  (143)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the Mly113I recognition sequence.
NarI  (143)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (142)
1 site
G G C G C C C C G C G G
SspDI  (142)
1 site
G G C G C C C C G C G G
AccB7I  (120)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different AccB7I sites may not be compatible.
AlwNI  (120)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
CaiI  (120)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different CaiI sites may not be compatible.
PflMI  (120)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
Van91I  (120)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different Van91I sites may not be compatible.
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
FbaI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
Ksp22I  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
Start  (0)
0 sites
hmAzami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
Product: humanized monomeric Azami-Green fluorescent protein
mammalian codon-optimized
hmAzami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
Product: humanized monomeric Azami-Green fluorescent protein
mammalian codon-optimized
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  26.0 kDa
ORF:  3 .. 680  =  678 bp
ORF:  226 amino acids  =  25.9 kDa  (no start codon)
ORF:  1 .. 681  =  681 bp
ORF:  227 amino acids  =  22.5 kDa  (no start codon)
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