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Plasmid Files

hmKikGR1

Humanized monomeric photoconvertible Kikume green-to-red fluorescent protein.

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hmKikGR1 Sequence and MaphmKikGR1.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (702) HaeII - PluTI (680) SfoI (678) BsaHI - NarI (677) KasI (676) StyI (650) BsiHKAI (647) DraIII (582) BsgI (580) BstXI (555) BpmI (520) BmgBI (480) BbsI (421) AleI (352) MscI * (310) TaqII (127) BtgI (119) BaeGI - Bme1580I (112) FseI (99) NaeI (97) BsiEI (96) NgoMIV (95) EagI (93) BbvCI - Bpu10I (37) Start (0) hmKikGR1 702 bp
End  (702)
0 sites
HaeII  (680)
1 site
R G C G C Y Y C G C G R
PluTI  (680)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (678)
1 site
G G C G C C C C G C G G
BsaHI  (677)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (677)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (676)
1 site
G G C G C C C C G C G G
StyI  (650)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsiHKAI  (647)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsgI  (580)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (555)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BpmI  (520)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmgBI  (480)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AleI  (352)
1 site
C A C N N N N G T G G T G N N N N C A C
MscI  (310)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
TaqII  (127)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BtgI  (119)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BaeGI  (112)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (112)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
FseI  (99)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (97)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BsiEI  (96)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
NgoMIV  (95)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
EagI  (93)
1 site
C G G C C G G C C G G C
BbvCI  (37)
1 site
C C T C A G C G G A G T C G
Bpu10I  (37)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Start  (0)
0 sites
hmKikGR1
1 .. 702  =  702 bp
233 amino acids  =  26.6 kDa
Product: humanized monomeric photoconvertible
Kikume green-to-red fluorescent protein
mammalian codon-optimized
hmKikGR1
1 .. 702  =  702 bp
233 amino acids  =  26.6 kDa
Product: humanized monomeric photoconvertible
Kikume green-to-red fluorescent protein
mammalian codon-optimized
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