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Plasmid Files

iRFP670

Near-infrared fluorescent protein with an emission peak at 670 nm, engineered from a bacterial phytochrome.

 
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 800 600 400 200 End (936) BsgI (889) BclI * (821) ApoI (748) AarI - BfuAI - BspMI (728) PluTI (695) SfoI (693) NarI (692) KasI (691) BsiHKAI (680) BaeGI - Bme1580I (674) TatI (611) PaeR7I - SmlI - XhoI (550) PfoI (514) DraIII (483) PstI (461) SfcI (457) NmeAIII (450) AcuI - Eco57MI (436) EarI (422) BssHII (400) BspDI * - ClaI * (328) MslI (314) Bts α I (304) SacII (160) BtgI (157) NaeI (132) NgoMIV (130) BmtI (85) NheI (81) BsmI (59) NruI * (35) AsiSI - PvuI (33) Start (0) iRFP670 936 bp
End  (936)
0 sites
BsgI  (889)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BclI  (821)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
ApoI  (748)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
AarI  (728)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BfuAI  (728)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (728)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PluTI  (695)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (693)
1 site
G G C G C C C C G C G G
NarI  (692)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (691)
1 site
G G C G C C C C G C G G
BsiHKAI  (680)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BaeGI  (674)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (674)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
TatI  (611)
1 site
W G T A C W W C A T G W
PaeR7I  (550)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
SmlI  (550)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (550)
1 site
C T C G A G G A G C T C
PfoI  (514)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
DraIII  (483)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PstI  (461)
1 site
C T G C A G G A C G T C
SfcI  (457)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
NmeAIII  (450)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AcuI  (436)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (436)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (422)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BssHII  (400)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BspDI  (328)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (328)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
MslI  (314)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtsαI  (304)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
SacII  (160)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BtgI  (157)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NaeI  (132)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (130)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BmtI  (85)
1 site
G C T A G C C G A T C G
NheI  (81)
1 site
G C T A G C C G A T C G
BsmI  (59)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NruI  (35)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
AsiSI  (33)
1 site
G C G A T C G C C G C T A G C G
PvuI  (33)
1 site
C G A T C G G C T A G C
Start  (0)
0 sites
iRFP670
1 .. 936  =  936 bp
311 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a
bacterial phytochrome (Shcherbakova and
Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP6
iRFP670
1 .. 936  =  936 bp
311 amino acids  =  34.5 kDa
Product: near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a
bacterial phytochrome (Shcherbakova and
Verkhusha, 2013)
derived from Rhodopseudomonas palustris RpBphP6
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