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Plasmid Files

mKO2

Monomeric Kusabira-Orange 2 fluorescent protein.

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mKO2 Sequence and MapmKO2.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (657) BfuAI - BspMI (618) BtgZI (574) BsaI (566) BbsI (535) BmrI (380) HincII - HpaI (378) ApoI (364) FspI - FspAI (323) BstBI (293) MmeI - StuI (266) EarI (221) BsaAI - PmlI (186) AhdI (177) NmeAIII (167) MluI (129) MfeI (74) DrdI (52) Start (0) mKO2 657 bp
End  (657)
0 sites
BfuAI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (618)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BtgZI  (574)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaI  (566)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BbsI  (535)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmrI  (380)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
HincII  (378)
1 site
G T Y R A C C A R Y T G
HpaI  (378)
1 site
G T T A A C C A A T T G
ApoI  (364)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
FspI  (323)
1 site
T G C G C A A C G C G T
FspAI  (323)
1 site
R T G C G C A Y Y A C G C G T R
BstBI  (293)
1 site
T T C G A A A A G C T T
MmeI  (266)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
StuI  (266)
1 site
A G G C C T T C C G G A
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
PmlI  (186)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NmeAIII  (167)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MluI  (129)
1 site
A C G C G T T G C G C A
MfeI  (74)
1 site
C A A T T G G T T A A C
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Start  (0)
0 sites
mKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange 2 fluorescent
protein
mKO2
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange 2 fluorescent
protein
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