Resources
Plasmid Files

mUkG1

Monomeric variant of Umikinoko-Green fluorescent protein.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

mUkG1 Sequence and MapmUkG1.dna
Map and Sequence File   
Sequence Author:  MBL International
Download Free Trial Get SnapGene Viewer

 600 400 200 mUkG1 End (684) BstBI (676) BpuEI (615) SmlI (594) BglII - BstYI (590) BsgI (571) AleI (512) MscI (503) EaeI (501) HincII - HpaI (474) TaqII (454) BsaAI (444) ApoI (353) BbsI (296) BmeT110I (254) AvaI - BsoBI (253) DraI (122) NsiI (69) NdeI (41) Start (0) mUkG1 684 bp
End  (684)
0 sites
BstBI  (676)
1 site
T T C G A A A A G C T T
BpuEI  (615)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SmlI  (594)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BglII  (590)
1 site
A G A T C T T C T A G A
BstYI  (590)
1 site
R G A T C Y Y C T A G R
BsgI  (571)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (512)
1 site
C A C N N N N G T G G T G N N N N C A C
MscI  (503)
1 site
T G G C C A A C C G G T
EaeI  (501)
1 site
Y G G C C R R C C G G Y
HincII  (474)
1 site
G T Y R A C C A R Y T G
HpaI  (474)
1 site
G T T A A C C A A T T G
TaqII  (454)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BsaAI  (444)
1 site
Y A C G T R R T G C A Y
ApoI  (353)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BbsI  (296)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmeT110I  (254)
1 site
C Y C G R G G R G C Y C
AvaI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
DraI  (122)
1 site
T T T A A A A A A T T T
NsiI  (69)
1 site
A T G C A T T A C G T A
NdeI  (41)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
Start  (0)
0 sites
mUkG1
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: monomeric variant of Umikinoko-Green
fluorescent protein
mUkG1
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: monomeric variant of Umikinoko-Green
fluorescent protein
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter