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pDsRed-Monomer-C In-Fusion® Ready

Linearized vector for fusing DsRed-Monomer to the N-terminus of a partner protein.

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pDsRed-Monomer-C In-Fusion Ready Sequence and MappDsRed-Monomer-C In-Fusion Ready.dna
Map and Sequence File   
Sequence Author:  Clontech
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 PciI (3301) ApaLI (2987) EcoO109I (2481) BsaI (2372) PfoI (2158) BstBI (2065) RsrII (1899) BsrDI (1616) PflFI - Tth111I (1501) MscI (1465) PluTI (1386) SfoI (1384) NarI (1383) KasI (1382) EagI (1289) BspDI * - ClaI * (1223) SfiI (1158) AseI (3366) NdeI (3593) SnaBI (3699) NheI (3950) BmtI (3954) AgeI (3959) FspAI (4020) BstEII (4112) AhdI (4151) AvaI - BsoBI (4233) BmeT110I (4234) SbfI (4312) BbsI (4392) BglII (4450) BsrGI (4510) PflMI (4553) AleI (4599) BspEI (4647) AflII (4653) End (4669) Start (0) EcoRI (8) BamHI (18) XbaI * (30) BclI * (40) MfeI (133) HincII - HpaI (146) Bts α I (222) MluI (269) SexAI * (972) pDsRed-Monomer-C In-Fusion® Ready 4669 bp
PciI  (3301)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (2987)
1 site
G T G C A C C A C G T G
EcoO109I  (2481)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (2372)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2158)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2065)
1 site
T T C G A A A A G C T T
RsrII  (1899)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (1616)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (1501)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1501)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (1465)
1 site
T G G C C A A C C G G T
PluTI  (1386)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (1384)
1 site
G G C G C C C C G C G G
NarI  (1383)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (1382)
1 site
G G C G C C C C G C G G
EagI  (1289)
1 site
C G G C C G G C C G G C
BspDI  (1223)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1223)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (1158)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (3366)
1 site
A T T A A T T A A T T A
NdeI  (3593)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (3699)
1 site
T A C G T A A T G C A T
NheI  (3950)
1 site
G C T A G C C G A T C G
BmtI  (3954)
1 site
G C T A G C C G A T C G
AgeI  (3959)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
FspAI  (4020)
1 site
R T G C G C A Y Y A C G C G T R
BstEII  (4112)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (4151)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AvaI  (4233)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (4233)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmeT110I  (4234)
1 site
C Y C G R G G R G C Y C
SbfI  (4312)
1 site
C C T G C A G G G G A C G T C C
BbsI  (4392)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (4450)
1 site
A G A T C T T C T A G A
BsrGI  (4510)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PflMI  (4553)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (4599)
1 site
C A C N N N N G T G G T G N N N N C A C
BspEI  (4647)
1 site
T C C G G A A G G C C T
AflII  (4653)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
End  (4669)
0 sites
Start  (0)
0 sites
EcoRI  (8)
1 site
G A A T T C C T T A A G
BamHI  (18)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (30)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (40)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (133)
1 site
C A A T T G G T T A A C
HincII  (146)
1 site
G T Y R A C C A R Y T G
HpaI  (146)
1 site
G T T A A C C A A T T G
BtsαI  (222)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
MluI  (269)
1 site
A C G C G T T G C G C A
SexAI  (972)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
1255 .. 2049  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
NeoR/KanR
1255 .. 2049  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin)
DsRed-Monomer
3972 .. 4646  =  675 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent
protein
mammalian codon-optimized
DsRed-Monomer
3972 .. 4646  =  675 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent
protein
mammalian codon-optimized
ori
2657 .. 3245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2657 .. 3245  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
275 .. 730  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
275 .. 730  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SV40 promoter
863 .. 1220  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
863 .. 1220  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
3420 .. 3723  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3420 .. 3723  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
3724 .. 3927  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
3724 .. 3927  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 poly(A) signal
147 .. 268  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
147 .. 268  =  122 bp
SV40 polyadenylation signal
AmpR promoter
757 .. 861  =  105 bp
AmpR promoter
757 .. 861  =  105 bp
HSV TK poly(A) signal
2281 .. 2328  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
2281 .. 2328  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
stop codons
32 .. 42  =  11 bp
stop codons in all three reading frames
stop codons
32 .. 42  =  11 bp
stop codons in all three reading frames
SV40 ori
1071 .. 1206  =  136 bp
SV40 origin of replication
SV40 ori
1071 .. 1206  =  136 bp
SV40 origin of replication
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