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Plasmid Files

pDsRed-Monomer-N1

Vector for fusing DsRed-Monomer to the C-terminus of a partner protein.

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pDsRed-Monomer-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4633) ApaLI (4319) EcoO109I (3813) BsaI (3704) PfoI (3490) RsrII (3231) BsrDI (2948) PflFI - Tth111I (2833) MscI (2797) PluTI (2718) SfoI (2716) NarI (2715) KasI (2714) BspDI * - ClaI * (2555) SfiI (2490) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) FspAI (727) BstEII (819) AhdI (858) SbfI (1019) BbsI (1099) BsrGI (1217) PflMI (1260) AleI (1306) NotI (1359) XbaI * (1369) MfeI (1465) HpaI (1478) Bts α I (1554) AflII (1597) SexAI * (2304) pDsRed-Monomer-N1 4691 bp
AflIII  (4633)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4633)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4319)
1 site
G T G C A C C A C G T G
EcoO109I  (3813)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3704)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3490)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3231)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2948)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2833)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2833)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (2797)
1 site
T G G C C A A C C G G T
PluTI  (2718)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2716)
1 site
G G C G C C C C G C G G
NarI  (2715)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2714)
1 site
G G C G C C C C G C G G
BspDI  (2555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2490)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
PaeR7I  (613)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (613)
1 site
C T C G A G G A G C T C
Eco53kI  (618)
1 site
G A G C T C C T C G A G
SacI  (620)
1 site
G A G C T C C T C G A G