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Plasmid Files

pDsRed2-C1

Vector for fusing Dsred2 to the N-terminus of a partner protein.

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pDsRed2-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4622) ApaLI (4308) PfoI (3479) RsrII (3220) BsrDI (2937) PflFI - Tth111I (2822) PluTI (2707) SfoI (2705) NarI (2704) KasI (2703) EagI (2610) BspDI * - ClaI * (2544) SfiI (2479) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) FspAI (661) BstEII (753) AhdI (792) SbfI (953) BbsI (1033) BstXI (1155) BsgI (1192) AleI (1240) BglII (1288) PaeR7I - XhoI (1292) Eco53kI (1297) SacI (1299) HindIII (1301) EcoRI (1308) SalI (1318) AccI (1319) Acc65I (1324) KpnI (1328) SacII (1331) PspOMI (1332) TspMI - XmaI (1335) ApaI (1336) SmaI (1337) BamHI (1339) XbaI * (1351) BclI * (1361) MfeI (1454) HpaI (1467) Bts α I (1543) MluI (1590) DraIII (1820) pDsRed2-C1 4680 bp
PciI  (4622)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4308)
1 site
G T G C A C C A C G T G
PfoI  (3479)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3220)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2937)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2707)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2705)
1 site
G G C G C C C C G C G G
NarI  (2704)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2703)
1 site
G G C G C C C C G C G G
EagI  (2610)
1 site
C G G C C G G C C G G C
BspDI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2479)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
FspAI  (661)
1 site
R T G C G C A Y Y A C G C G T R
BstEII  (753)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (792)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (953)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1033)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BstXI  (1155)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsgI  (1192)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (1240)
1 site
C A C N