Sticky ends from different EcoO109I sites may not be compatible.
BsaI (3744) 1 site
GGTCTCNCCAGAGN(N)4
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.
RsrII (3271) 1 site
CGGWCCGGCCWGGC
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BsrDI (2988) 1 site
GCAATGNNCGTTAC
Sticky ends from different BsrDI sites may not be compatible.
PflFI (2873) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (2873) 1 site
GACNNNGTCCTGNNNCAG
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
FspI (2857) 1 site
TGCGCAACGCGT
MscI (2837) 1 site
TGGCCAACCGGT
PluTI (2758) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI (2756) 1 site
GGCGCCCCGCGG
NarI (2755) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI (2754) 1 site
GGCGCCCCGCGG
EagI (2661) 1 site
CGGCCGGCCGGC
BspDI (2595) 1 site
ATCGATTAGCTA
* Blocked by Dam methylation.
ClaI (2595) 1 site
ATCGATTAGCTA
* Blocked by Dam methylation.
StuI (2576) 1 site
AGGCCTTCCGGA
SfiI (2530) 1 site
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI (7) 1 site
ATTAATTAATTA
NdeI (234) 1 site
CATATGGTATAC
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI (340) 1 site
TACGTAATGCAT
NheI (591) 1 site
GCTAGCCGATCG
BmtI (595) 1 site
GCTAGCCGATCG
AfeI (596) 1 site
AGCGCTTCGCGA
AgeI (600) 1 site
ACCGGTTGGCCA
BmgBI (678) 1 site
CACGTCGTGCAG
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstEII (795) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.
XmnI (859) 1 site
GAANNNNTTCCTTNNNNAAG
Bpu10I (1216) 1 site
CCTNAGCGGANTCG
Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible.
BspEI (1330) 1 site
TCCGGAAGGCCT
BglII (1339) 1 site
AGATCTTCTAGA
PaeR7I (1343) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI (1343) 1 site
CTCGAGGAGCTC
Eco53kI (1348) 1 site
GAGCTCCTCGAG
SacI (1350) 1 site
GAGCTCCTCGAG
HindIII (1352) 1 site
AAGCTTTTCGAA
EcoRI (1359) 1 site
GAATTCCTTAAG
SalI (1369) 1 site
GTCGACCAGCTG
Acc65I (1375) 1 site
GGTACCCCATGG
KpnI (1379) 1 site
GGTACCCCATGG
PspOMI (1383) 1 site
GGGCCCCCCGGG
TspMI (1386) 1 site
CCCGGGGGGCCC
XmaI (1386) 1 site
CCCGGGGGGCCC
Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI (1387) 1 site
GGGCCCCCCGGG
ApaI can be used between 25°C and 37°C.
SmaI (1388) 1 site
CCCGGGGGGCCC
SmaI can be used at 37°C for brief incubations.
BamHI (1390) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.