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Plasmid Files

pTurboYFP-C

Vector for fusing TurboYFP to the N-terminus of a partner protein.

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pTurboYFP-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PciI (4685) EcoO109I (3865) PfoI (3542) RsrII (3283) PflFI - Tth111I (2885) FspI (2869) EagI (2673) BspDI * - ClaI * (2607) StuI (2588) BseRI (2585) SfiI (2542) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) SgrAI (760) AleI (790) XmnI (850) PflMI (1014) BlpI (1231) BssHII (1284) BspEI (1327) BglII (1351) PaeR7I - XhoI (1355) Eco53kI (1360) SacI (1362) HindIII (1364) EcoRI (1371) PstI (1380) SalI (1381) AccI (1382) Acc65I (1387) KpnI (1391) PspOMI (1395) TspMI - XmaI (1398) ApaI (1399) SmaI (1400) BamHI (1402) XbaI * (1414) BclI * (1424) MfeI (1517) HpaI (1530) MluI (1653) DraIII (1883) SexAI * (2356) pTurboYFP-C 4743 bp
PciI  (4685)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3865)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3542)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3283)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (2885)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2885)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2869)
1 site
T G C G C A A C G C G T
EagI  (2673)
1 site
C G G C C G G C C G G C
BspDI  (2607)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2607)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2588)
1 site
A G G C C T T C C G G A
BseRI  (2585)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2542)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
SgrAI  (760)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AleI  (790)
1 site
C A C N N N N G T G G T G N N N N C A C
XmnI  (850)
1 site
G A A N N N N T T C C T T N N N N A A G
PflMI  (1014)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BlpI  (1231)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BssHII  (1284)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BspEI  (1327)
1 site
T C C G G A A G G C C T
BglII  (1351)
1 site
A G A T C T T C T A G A
PaeR7I  (1355)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1355)
1 site
C T C G A G G A G C T C
Eco53kI  (1360)
1 site
G A G C T C C T C G A G
SacI  (1362)
1 site
G A G C T C C T C G A G
HindIII  (1364)
1 site
A A G C T T T T C G A A
EcoRI  (1371)
1 site
G A A T T C C T T A A G
PstI  (1380)
1 site
C T G C A G G A C G T C
SalI  (1381)
1 site
G T C G A C C A G C T G
AccI  (1382)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1387)
1 site
G G T A C C C C A T G G
KpnI  (1391)
1 site
G G T A C C C C A T G G
PspOMI  (1395)
1 site
G G G C C C C C C G G G
TspMI  (1398)
1 site
C C C G G G G G G C C C
XmaI  (1398)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1399)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1400)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1402)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1414)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1424)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1517)
1 site
C A A T T G G T T A A C
HpaI  (1530)
1 site
G T T A A C C A A T T G
MluI  (1653)
1 site
A C G C G