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Plasmid Files

pmCherry-C1

Vector for fusing mCherry to the N-terminus of a partner protein.

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pmCherry-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4664) EcoO109I (3844) BsaI (3735) PfoI (3521) RsrII (3262) BsrDI (2979) PflFI - Tth111I (2864) FspI (2848) EagI (2652) BspDI * - ClaI * (2586) SfiI (2521) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) AhdI (807) SbfI (968) BbsI (1048) PflMI (1052) BbvCI - Bpu10I (1151) BsgI (1207) SgrAI (1294) XcmI (1299) BsrGI (1313) BspEI (1321) BglII (1330) PaeR7I - XhoI (1334) Eco53kI (1339) SacI (1341) HindIII (1343) EcoRI (1350) SalI (1360) AccI (1361) Acc65I (1366) KpnI (1370) SacII (1373) PspOMI (1374) TspMI - XmaI (1377) ApaI (1378) SmaI (1379) BamHI (1381) XbaI * (1393) BclI * (1403) MfeI (1496) HpaI (1509) Bts α I (1585) MluI (1632) DraIII (1862) SexAI * (2335) pmCherry-C1 4722 bp
PciI  (4664)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3844)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3735)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3521)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3262)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2979)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2864)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2864)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2848)
1 site
T G C G C A A C G C G T
EagI  (2652)
1 site
C G G C C G G C C G G C
BspDI  (2586)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2586)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2521)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
AhdI  (807)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (968)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1048)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1052)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BbvCI  (1151)
1 site
C C T C A G C G G A