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pSPORT 2

Vector for cloning, sequencing, and bacterial expression of cDNAs.

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pSPORT 2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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ZraI (193) NaeI (4181) NgoMIV (4179) DraIII (4078) PsiI (3950) XmnI (3378) ScaI (3259) TatI (3257) BsaI (2840) AhdI (2779) AlwNI (2260) AatII (195) SphI (201) MluI (203) BsiWI (206) SnaBI (210) HindIII (213) BamHI (219) XbaI (225) SalI (231) AccI (232) Eco53kI (239) SacI (241) SpeI (243) EagI - NotI (254) AvaI - BsoBI - TspMI - XmaI (261) BmeT110I (262) SmaI (263) EcoRI (266) BspEI (270) RsrII (273) AgeI (275) Acc65I (278) KpnI (282) PstI - SbfI (287) BfuAI - BspMI (290) KasI (555) NarI * (556) SfoI (557) PluTI (559) BsmBI (579) HpaI (692) EcoRV (748) PspOMI (987) ApaI (991) BstEII (1012) BclI * (1180) MauBI (1651) PciI (1844) pSPORT 2 4310 bp
ZraI  (193)
1 site
G A C G T C C T G C A G
NaeI  (4181)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4179)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (4078)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (3950)
1 site
T T A T A A A A T A T T
XmnI  (3378)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3259)
1 site
A G T A C T T C A T G A
TatI  (3257)
1 site
W G T A C W W C A T G W
BsaI  (2840)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2779)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2260)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AatII  (195)
1 site
G A C G T C C T G C A G
SphI  (201)
1 site
G C A T G C C G T A C G
MluI  (203)
1 site
A C G C G T T G C G C A
BsiWI  (206)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (210)
1 site
T A C G T A A T G C A T
HindIII  (213)
1 site
A A G C T T T T C G A A
BamHI  (219)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (225)
1 site
T C T A G A A G A T C T
SalI  (231)
1 site
G T C G A C C A G C T G
AccI  (232)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Eco53kI  (239)
1 site
G A G C T C C T C G A G
SacI  (241)
1 site
G A G C T C C T C G A G
SpeI  (243)
1 site
A C T A G T T G A T C A
EagI  (254)
1 site
C G G C C G G C C G G C
NotI  (254)
1 site
G C G G C C G C C G C C G G C G
AvaI  (261)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (261)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (261)
1 site
C C C G G G G G G C C C
XmaI  (261)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (262)
1 site
C Y C G R G G R G C Y C
SmaI  (263)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (266)
1 site
G A A T T C C T T A A G
BspEI  (270)
1 site
T C C G G A A G G C C T
RsrII  (273)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (275)
1 site
A C C G G T T G G C C A
Acc65I  (278)
1 site
G G T A C C C C A T G G
KpnI  (282)
1 site
G G T A C C C C A T G G
PstI  (287)
1 site
C T G C A G G A C G T C
SbfI  (287)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (290)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4