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Plasmid Files

pAcMP3

Baculovirus transfer vector for expressing proteins with the BD BaculoGold™ system using the basic protein promoter. For an alternative MCS, use pAcMP2.

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pAcMP3 Sequence and MappAcMP3.dna
Map and Sequence File   
Sequence Author:  BD Biosciences
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 NdeI (9638) ScaI (8947) BpmI (8537) AlwNI (7990) PspFI (7882) BseYI (7878) BspQI - SapI (7458) FspAI (6541) SgrDI (6388) SgrAI (6119) SnaBI (5031) PasI (486) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) StyI (1501) PaeR7I - PspXI - XhoI (1901) SphI (2135) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) BamHI (4342) XbaI (4357) EcoRI (4361) EagI - NotI (4372) MCS BglII - PstI (4382) KflI - PpuMI (4400) pAcMP3 9852 bp
NdeI  (9638)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
ScaI  (8947)
1 site
A G T A C T T C A T G A
BpmI  (8537)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AlwNI  (7990)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7882)
1 site
C C C A G C G G G T C G
BseYI  (7878)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BspQI  (7458)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7458)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
FspAI  (6541)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6388)
1 site
C G T C G A C G G C A G C T G C
SgrAI  (6119)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SnaBI  (5031)
1 site
T A C G T A A T G C A T
PasI  (486)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PacI  (583)
1 site
T T A A T T A A A A T T A A T T
XcmI  (746)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BtgI  (868)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (871)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstEII  (923)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1395)
1 site
G G G C C C C C C G G G
ApaI  (1399)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (1399)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
StyI  (1501)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
PaeR7I  (1901)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1901)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1901)
1 site
C T C G A G G A G C T C
SphI  (2135)
1 site
G C A T G C C G T A C G
BbvCI  (3405)
1 site
C C T C A G C G G A G T C G
Bpu10I  (3405)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NgoMIV  (3770)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
NaeI  (3772)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BamHI  (4342)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (4357)
1 site
T C T A G A A G A T C T
EcoRI  (4361)
1 site
G A A T T C C T T A A G
EagI  (4372)
1 site
C G G C C G G C C G G C
NotI  (4372)
1 site
G C G G C C G C C G C C G G C G
BglII  (4382)
1 site
A G A T C T T C T A G A
PstI  (4382)
1 site
C T G C A G G A C G T C
KflI  (4400)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (4400)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
AmpR
8394 .. 9254  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   8394 .. 9185  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
8394 .. 9254  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   9186 .. 9254  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
8394 .. 9254  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
4867 .. 5572  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
4867 .. 5572  =  706 bp
contains part of ORF1629
ori
7635 .. 8223  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
7635 .. 8223  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
basic protein promoter
4017 .. 4341  =  325 bp
baculovirus basic protein promoter, which allows
expression during the late instead of very late phase
of viral infection
basic protein promoter
4017 .. 4341  =  325 bp
baculovirus basic protein promoter, which allows
expression during the late instead of very late phase
of viral infection
AmpR promoter
9255 .. 9359  =  105 bp
AmpR promoter
9255 .. 9359  =  105 bp
MCS
4342 .. 4405  =  64 bp
multiple cloning site
MCS
4342 .. 4405  =  64 bp
multiple cloning site
ORF603
3332 .. 3937  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3332 .. 3937  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
4984 .. 5572  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
4984 .. 5572  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3170 .. 3294  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3170 .. 3294  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
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