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pAcSG2

Compact baculovirus transfer vector for expressing proteins using the polyhedrin promoter, for use with the BD BaculoGold™ system.

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pAcSG2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  BD Biosciences
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PfoI (5404) EcoO109I (5347) ScaI (4851) PvuI (4741) NmeAIII (4519) BglI (4491) BpmI (4441) BsaI (4432) BmrI (4411) AlwNI (3894) PspFI (3786) BseYI (3782) BspQI - SapI (3362) HpaI (3100) BspEI (3042) BstBI (2948) NgoMIV (361) NaeI (363) EcoRV (591) PaeR7I - XhoI (690) EcoRI (696) StuI (704) BtgI - NcoI - StyI (708) Eco53kI (716) BanII - SacI (718) EagI - NotI (721) PstI - SbfI (732) Acc65I (734) KpnI (738) TspMI - XmaI (740) SmaI (742) BglII (746) BsaAI - SnaBI (935) SwaI (1025) BmgBI (1439) BtgZI (1568) AgeI (1783) BspDI - ClaI (1907) SgrAI (2023) SalI - SgrDI (2292) AccI (2293) FspAI (2445) MscI (2515) pAcSG2 5544 bp
PfoI  (5404)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (5347)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
ScaI  (4851)
1 site
A G T A C T T C A T G A
PvuI  (4741)
1 site
C G A T C G G C T A G C
NmeAIII  (4519)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (4491)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BpmI  (4441)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (4432)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (4411)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AlwNI  (3894)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3786)
1 site
C C C A G C G G G T C G
BseYI  (3782)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (3362)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3362)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
HpaI  (3100)
1 site
G T T A A C C A A T T G
BspEI  (3042)
1 site
T C C G G A A G G C C T
BstBI  (2948)
1 site
T T C G A A A A G C T T
NgoMIV  (361)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (363)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
EcoRV  (591)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaeR7I  (690)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (690)
1 site
C T C G A G G A G C T C
EcoRI  (696)
1 site
G A A T T C C T T A A G
StuI  (704)
1 site
A G G C C T T C C G G A
BtgI  (708)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (708)
1 site
C C A T G G G G T A C C
StyI  (708)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
Eco53kI  (716)
1 site
G A G C T C C T C G A G
BanII  (718)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (718)
1 site
G A G C T C C T C G A G
EagI  (721)
1 site
C G G C C G G C C G G C
NotI  (721)
1 site
G C G G C C G C C G C C G G C G
PstI  (732)
1 site
C T G C A G G A C G T C
SbfI  (732)
1 site
C C T G C A G G G G A C G T C C
Acc65I  (734)
1 site
G G T A C C C C A T G G
KpnI  (738)
1 site
G G T A C C C C A T G G
TspMI  (740)
1 site
C C C G G G G G G C C C
XmaI  (740)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (742)
1 site
C C C G G G G G