pTriEx-6

Insect, bacterial, and mammalian vector, encoding a N-terminal Strep-Tag® II-HRV 3C cassette and a cleavable C-terminal 10xHis tag.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
NcoI (2112) BstBI (2139) EcoNI (2165) SanDI (2171) TspMI - XmaI (2174) SmaI (2176) Acc65I (2178) KpnI (2182) BamHI (2185) EagI - NotI (2205) Eco53kI (2215) SacI (2217) DraIII (2278) Bsu36I (2288) pTriEx™-6 5070 bp
NcoI  (2112)
1 site
C C A T G G G G T A C C
BstBI  (2139)
1 site
T T C G A A A A G C T T
EcoNI  (2165)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SanDI  (2171)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
TspMI  (2174)
1 site
C C C G G G G G G C C C
XmaI  (2174)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2176)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Acc65I  (2178)
1 site
G G T A C C C C A T G G
KpnI  (2182)
1 site
G G T A C C C C A T G G
BamHI  (2185)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EagI  (2205)
1 site
C G G C C G G C C G G C
NotI  (2205)
1 site
G C G G C C G C C G C C G G C G
Eco53kI  (2215)
1 site
G A G C T C C T C G A G
SacI  (2217)
1 site
G A G C T C C T C G A G
DraIII  (2278)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Bsu36I  (2288)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4148 .. 4939  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4940 .. 5008  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Exon 1
1597 .. 1698  =  102 bp
Exon 1
1597 .. 1698  =  102 bp
intron
1699 .. 1862  =  164 bp
intron
1699 .. 1862  =  164 bp
Exon 2
1863 .. 2454  =  592 bp
Exon 2
1863 .. 2454  =  592 bp
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
2633 .. 3338  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
2633 .. 3338  =  706 bp
contains part of ORF1629
ori
3548 .. 4135  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3548 .. 4135  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
2573 .. 2620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2573 .. 2620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
2750 .. 3338  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
2750 .. 3338  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
HRV 3C site
2153 .. 2176  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
HRV 3C site
2153 .. 2176  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
thrombin site
2222 .. 2239  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
thrombin site
2222 .. 2239  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
10xHis
2246 .. 2275  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
2246 .. 2275  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
2452 .. 2563  =  112 bp
rabbit globin terminator
2452 .. 2563  =  112 bp
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
2101 .. 2106  =  6 bp
ribosome binding site
RBS
2101 .. 2106  =  6 bp
ribosome binding site
poly(A) signal
2430 .. 2435  =  6 bp
poly(A) signal
2430 .. 2435  =  6 bp
MCS
2174 .. 2218  =  45 bp
multiple cloning site
MCS
2174 .. 2218  =  45 bp
multiple cloning site
ORF:  4278 .. 4544  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5058 .. 305  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  343 .. 948  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  387 .. 617  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  1587 .. 1841  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
ORF:  2081 .. 2416  =  336 bp
ORF:  111 amino acids  =  12.3 kDa
ORF:  2750 .. 3367  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  4148 .. 5008  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTriEx-6.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.