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pFN26A (BIND) hRluc-neo Flexi® Vector

Flexi® Vector encoding Renilla luciferase fused to a neomycin resistance gene. This vector is useful for testing transcriptional activation domains.

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pFN26A (BIND) hRluc-neo Flexi Vector.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BglII (7065) MluI (6946) PciI (5969) AhdI (5799) BspDI - ClaI (4754) BstXI (4694) RsrII (4489) NaeI (4475) NgoMIV (4473) BssHII (4370) PluTI (3976) SfoI (3974) NarI (3973) KasI (3972) EcoO109I (3709) PasI (3638) SpeI (152) NdeI (387) NheI (1052) BmtI (1056) PaeR7I - XhoI (1300) AsiSI - PvuI (1558) DraI - PmeI (1925) EcoRI (1930) XbaI (1957) SbfI (1973) BlpI (2038) NotI (2068) PsiI (2191) MfeI (2220) SexAI * (2565) SfiI (2751) StuI (2797) AvrII (2798) PflMI (2852) DraIII (3052) PfoI * (3144) EcoRV (3349) Bsu36I (3450) NruI (3525) pFN26A (BIND) hRluc-neo Flexi® Vector 7070 bp
BglII  (7065)
1 site
A G A T C T T C T A G A
MluI  (6946)
1 site
A C G C G T T G C G C A
PciI  (5969)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (5799)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspDI  (4754)
1 site
A T C G A T T A G C T A
ClaI  (4754)
1 site
A T C G A T T A G C T A
BstXI  (4694)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
RsrII  (4489)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (4475)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4473)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII  (4370)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PluTI  (3976)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3974)
1 site
G G C G C C C C G C G G
NarI  (3973)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3972)
1 site
G G C G C C C C G C G G
EcoO109I  (3709)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PasI  (3638)
1 site
C C