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Plasmid Files

pGL2-Enhancer

SV40 enhancer-containing vector for measuring the activity of promoter sequences with a luciferase assay.

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pGL2-Enhancer.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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Acc65I (8) SmaI (3) TspMI - XmaI (1) GLprimer1 (5822 .. 5844) DraIII (5297) XmnI (4744) ScaI (4625) EaeI (4533) NmeAIII (4293) BsaI (4206) AhdI (4145) AlwNI (3668) PciI (3252) AfeI (3128) RVprimer4 (3053 .. 3072) PshAI (3067) AccI (3003) SalI (3002) BamHI (2996) KpnI (12) Eco53kI (16) SacI (18) MluI (22) NheI (28) BmtI (32) PaeR7I - XhoI (33) BglII (37) HindIII (47) GLprimer2 (77 .. 99) KasI (108) NarI (109) SfoI (110) PluTI (112) XbaI (123) PfoI * (183) BsiWI (230) BsrGI (566) EcoRI (663) BstEII (683) Bsu36I (689) XcmI (811) EcoO109I - PpuMI (1255) PacI (1400) EcoRV (1414) BspDI - ClaI (1441) BfuAI - BspMI (1474) SgrAI (1504) EcoNI (1693) PflMI (2044) BsgI (2276) BsaBI * (2507) BtgI - NcoI (2748) SexAI * (2887) pGL2-Enhancer 5855 bp
Acc65I  (8)
1 site
G G T A C C C C A T G G
SmaI  (3)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (1)
1 site
C C C G G G G G G C C C
XmaI  (1)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
DraIII  (5297)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (4744)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4625)
1 site
A G T A C T T C A T G A
EaeI  (4533)
1 site
Y G G C C R R C C G G Y
NmeAIII  (4293)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaI  (4206)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4145)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3668)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3252)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AfeI  (3128)
1 site
A G C G C T T C G C G A
PshAI  (3067)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AccI  (3003)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (3002)
1 site
G T C G A C C A G C T G
BamHI  (2996)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (12)
1 site
G G T A C C C C A T G G
Eco53kI  (16)
1 site
G A G C T C C T C G A G
SacI  (18)
1 site
G A G C T C C T C G A G
MluI  (22)
1 site
A C G C G T T G C G C A
NheI  (28)
1 site
G C T A G C C G A T C G
BmtI  (32)
1 site
G C T A G C C G A T C G
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (33)
1 site
C T C G A G G A G C T C
BglII  (37)
1 site
A G A T C T T C T A G A
HindIII  (47)
1 site
A A G C T T T T C G A A
KasI  (108)
1 site
G G C G C C C C G C G G
NarI  (109)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (110)
1 site
G G C G C C C C G C G G
PluTI  (112)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XbaI  (123)
1 site
T C T A G A A G A T C T
PfoI  (183)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BsiWI  (230)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BsrGI  (566)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (663)
1 site
G A A T T C C T T A A G
BstEII  (683)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Bsu36I  (689)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
XcmI  (811)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoO109I  (1255)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (1255)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
PacI  (1400)
1 site
T T A A T T A A A A T T A A T T
EcoRV  (1414)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BspDI  (1441)
1 site
A T C G A T T A G C T A
ClaI  (1441)
1 site
A T C G A T T A G C T A
BfuAI  (1474)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1474)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SgrAI  (1504)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EcoNI  (1693)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PflMI  (2044)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsgI  (2276)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaBI  (2507)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgI  (2748)
1 site
C C R Y