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pGL4.84[hRlucCP/Puro]

Promoterless vector encoding highly destabilized Renilla luciferase for measuring the activity of promoter and enhancer sequences.

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pGL4.84[hRlucCP Puro].dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BsgI (4864) AarI - BfuAI - BspMI (4836) RVprimer3 (4815 .. 4834) BsmBI (4663) SpeI (4650) BstZ17I (4331) SacII (4215) PvuI (4191) AhdI (3821) BstEII (3746) BstXI (3743) AleI (3741) NotI (3719) BspHI (3619) ApaLI (3213) BciVI (3102) DrdI (3007) AflIII - PciI (2899) AfeI (2775) BbsI (2728) RVprimer4 (2700 .. 2719) SalI (2649) BstBI (2635) AgeI (2570) BglI - SfiI (8) Acc65I (14) KpnI (18) Bpu10I (19) Eco53kI (23) SacI (25) NheI (27) BmtI (31) AbsI - PaeR7I - PspXI - XhoI (33) BglII (46) BglI - SfiI (59) HindIII (65) BglI (100) PfoI * (380) BsaI (640) ZraI (795) PflFI - Tth111I (796) AatII (797) PasI (874) EcoRI (1032) AflII (1062) FseI (1238) AanI - AanI - PsiI (1359) HpaI (1379) MfeI (1388) BamHI (1481) FspI (1519) PvuII (1591) StuI (1914) BtgZI (2013) BlpI (2252) SgrAI (2420) pGL4.84[hRlucCP/Puro] 4866 bp
BsgI  (4864)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AarI  (4836)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (4836)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4836)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI  (4663)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SpeI  (4650)
1 site
A C T A G T T G A T C A
BstZ17I  (4331)
1 site
G T A T A C C A T A T G
SacII  (4215)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (4191)
1 site
C G A T C G G C T A G C
AhdI  (3821)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (3746)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (3743)
1 site
C C A N N N N N N T G