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Plasmid Files

pJAZZ®-OC

Linear bacterial vector with a chloramphenicol resistance gene for cloning DNA sequences that are unstable in circular plasmids.

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pJAZZ-OC Sequence and MappJAZZ-OC.dna
Map and Sequence File   
Sequence Author:  Lucigen
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 12,500 10,000 7500 5000 2500 telL CB lacZ α rrnB T1 terminator CmR telR N15 ori End (12,887) PacI (12,391) AanI - AanI (12,044) BglII (11,781) tonB terminator AvrII (11,729) NcoI (11,583) cat promoter NZ RevC (10,877 .. 10,898) SwaI (10,898) NotI (10,845) AhdI (10,836) MCS-R SmaI (10,821) PvuI (10,769) BsrBI (10,494) lac promoter BmtI (10,366) NheI (10,362) NaeI (10,355) NgoMIV (10,353) SmaI (10,333) ApaI (10,324) PspOMI (10,320) MCS-L AhdI (10,312) NotI (10,300) SpeI (10,293) SL1 (10,272 .. 10,292) T7Te terminator XbaI (10,207) AanI - AanI (9985) AanI - AanI (9948) RsrII (9656) BbvCI (8044) AanI - AanI (7664) BbeI (7282) SfoI (7280) NarI (7279) KasI (7278) PaeR7I - PspXI - XhoI (6350) BsrGI (5170) AanI - AanI (4980) AatII (4824) ZraI (4822) BstBI (4086) SexAI * (3996) MauBI (3125) SacI (1874) Eco53kI (1872) AarI (1785) PpuMI (1632) SgrDI (1402) AanI - AanI (1328) BstZ17I (1064) PmeI (956) AanI - AanI (728) Start (0) pJAZZ®-OC 12,887 bp
End  (12,887)
0 sites
PacI  (12,391)
1 site
T T A A T T A A A A T T A A T T
AanI  (12,044)
7 sites
T T A T A A A A T A T T
AanI  (12,044)
7 sites
T T A T A A A A T A T T
BglII  (11,781)
1 site
A G A T C T T C T A G A
AvrII  (11,729)
1 site
C C T A G G G G A T C C
NcoI  (11,583)
1 site
C C A T G G G G T A C C
SwaI  (10,898)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
NotI  (10,845)
2 sites
G C G G C C G C C G C C G G C G
AhdI  (10,836)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SmaI  (10,821)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
PvuI  (10,769)
1 site
C G A T C G G C T A G C
BsrBI  (10,494)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
BmtI  (10,366)
1 site
G C T A G C C G A T C G
NheI  (10,362)
1 site
G C T A G C C G A T C G
NaeI  (10,355)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (10,353)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
SmaI  (10,333)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
ApaI  (10,324)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (10,320)
1 site
G G G C C C C C C G G G
AhdI  (10,312)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (10,300)
2 sites
G C G G C C G C C G C C G G C G
SpeI  (10,293)
1 site
A C T A G T T G A T C A
XbaI  (10,207)
1 site
T C T A G A A G A T C T
AanI  (9985)
7 sites
T T A T A A A A T A T T
AanI  (9985)
7 sites
T T A T A A A A T A T T
AanI  (9948)
7 sites
T T A T A A A A T A T T
AanI  (9948)
7 sites
T T A T A A A A T A T T
RsrII  (9656)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BbvCI  (8044)
1 site
C C T C A G C G G A G T C G
AanI  (7664)
7 sites
T T A T A A A A T A T T
AanI  (7664)
7 sites
T T A T A A A A T A T T
BbeI  (7282)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the BbeI
recognition sequence.
SfoI  (7280)
1 site
G G C G C C C C G C G G
NarI  (7279)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (7278)
1 site
G G C G C C C C G C G G
PaeR7I  (6350)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (6350)
1 site
V C T C G A G B B G A G C T C V
XhoI  (6350)
1 site
C T C G A G G A G C T C
BsrGI  (5170)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (4980)
7 sites
T T A T A A A A T A T T
AanI  (4980)
7 sites
T T A T A A A A T A T T
AatII  (4824)
1 site
G A C G T C C T G C A G
ZraI  (4822)
1 site
G A C G T C C T G C A G
BstBI  (4086)
1 site
T T C G A A A A G C T T
SexAI  (3996)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MauBI  (3125)
1 site
C G C G C G C G G C G C G C G C
SacI  (1874)
1 site
G A G C T C C T C G A G
Eco53kI  (1872)
1 site
G A G C T C C T C G A G
AarI  (1785)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
PpuMI  (1632)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site
C G T C G A C G G C A G C T G C
AanI  (1328)
7 sites
T T A T A A A A T A T T
AanI  (1328)
7 sites
T T A T A A A A T A T T
BstZ17I  (1064)
1 site
G T A T A C C A T A T G
PmeI  (956)
1 site
G T T T A A A C C A A A T T T G
AanI  (728)
7 sites
T T A T A A A A T A T T
AanI  (728)
7 sites
T T A T A A A A T A T T
Start  (0)
0 sites
NZ RevC
22-mer  /  32% GC
1 binding site
10,877 .. 10,898  =  22 annealed bases
Tm  =  52°C
sequencing primer
SL1
21-mer  /  57% GC
1 binding site
10,272 .. 10,292  =  21 annealed bases
Tm  =  59°C
sequencing primer
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 1:  -35  
   10,450 .. 10,455  =  6 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 2:  
   10,456 .. 10,473  =  18 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
   Segment 3:  -10  
   10,474 .. 10,480  =  7 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
promoter for the E. coli lac operon
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
cat promoter
10,966 .. 11,068  =  103 bp
promoter of the E. coli cat gene
cat promoter
10,966 .. 11,068  =  103 bp
promoter of the E. coli cat gene
CmR
11,069 .. 11,728  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
11,069 .. 11,728  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
tonB terminator
11,742 .. 11,773  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
11,742 .. 11,773  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
telR
12,860 .. 12,887  =  28 bp
telomeric sequence from coliphage N15
telR
12,860 .. 12,887  =  28 bp
telomeric sequence from coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
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