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Plasmid Files

pJW168

Bacterial vector allowing inducible expression of Cre recombinase for in vivo site-specific recombination.

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pJW168.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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SmaI (5720) BmeT110I (5719) AvaI - BsoBI - TspMI - XmaI (5718) HindIII (5691) BmtI (5674) NheI (5670) BtgI - NcoI - StyI (5664) SpeI (4540) BseRI (4298) AflII (4249) EagI - NotI (4220) AhdI (3647) BsaI (3581) BglI (3529) FspI (3424) ScaI (3166) BssS α I (2978) EcoRI (5723) PshAI (199) Bpu10I (208) AsiSI (578) SexAI * (602) BstBI (623) RsrII (730) NruI (820) AgeI (989) Acc65I (1042) KpnI (1046) PstI (1051) XbaI (1053) StuI (1065) PfoI (1307) DrdI (1358) PflFI - Tth111I (1410) PflMI (1451) MluI (1869) BstEII (2050) PspOMI (2076) ApaI - BanII (2080) HpaI (2375) pJW168 5728 bp
SmaI  (5720)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BmeT110I  (5719)
1 site
C Y C G R G G R G C Y C
AvaI  (5718)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (5718)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (5718)
1 site
C C C G G G G G G C C C
XmaI  (5718)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
HindIII  (5691)
1 site
A A G C T T T T C G A A
BmtI  (5674)
1 site
G C T A G C C G A T C G
NheI  (5670)
1 site
G C T A G C C G A T C G
BtgI  (5664)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (5664)
1 site
C C A T G G G G T A C C
StyI  (5664)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
SpeI  (4540)
1 site
A C T A G T T G A T C A
BseRI  (4298)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AflII  (4249)
1 site
C T T A A G G A A T T C
EagI  (4220)
1 site
C G G C C G G C C G G C
NotI  (4220)
1 site
G C G G C C G C C G C C G G C G
AhdI  (3647)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (3581)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglI  (3529)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (3424)
1 site