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Plasmid Files

pRANGER™-BTB-1

Broad host range expression vector with an ampicillin resistance marker and with the pBBR1 replicon for Gram-negative bacteria.

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pRANGER-BTB-1 Sequence and MappRANGER-BTB-1.dna
Map and Sequence File   
Sequence Author:  Lucigen
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 EcoRI (3776) AflIII - MluI (3605) BstEII (3547) AgeI (3544) BspQI - EarI - SapI (3533) DrdI (3504) BspEI (3185) NruI (3178) SspI (3106) NspI (2838) AflII (2771) BsaBI (2711) AhdI (2418) BsaI (2352) AseI (2243) SfcI (2172) PvuI (2049) XcmI (2035) ScaI (1937) HincII (0) EcoRI (4) HindIII (10) SacII (103) PvuII (818) MscI (875) BbsI (1061) AfeI (1127) BclI * (1255) BseRI (1370) AlwNI - BstAPI (1450) PshAI (1511) SnaBI (1537) Bpu10I (1617) ApaLI (1746) XmnI (1818) pRANGER™-BTB-1 3784 bp
EcoRI  (3776)
2 sites
G A A T T C C T T A A G
AflIII  (3605)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (3605)
1 site
A C G C G T T G C G C A
BstEII  (3547)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AgeI  (3544)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BspQI  (3533)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (3533)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (3533)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
DrdI  (3504)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BspEI  (3185)
1 site
T C C G G A A G G C C T
NruI  (3178)
1 site
T C G C G A A G C G C T
SspI  (3106)
1 site
A A T A T T T T A T A A
NspI  (2838)
1 site
R C A T G Y Y G T A C R
AflII  (2771)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
BsaBI  (2711)
1 site
G A T N N N N A T C C T A N N N N T A G
AhdI  (2418)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (2352)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AseI  (2243)
1 site
A T T A A T T A A T T A
SfcI  (2172)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
PvuI  (2049)
1 site
C G A T C G G C T A G C
XcmI  (2035)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
ScaI  (1937)
1 site
A G T A C T T C A T G A
HincII  (0)
1 site
G T Y R A C C A R Y T G
EcoRI  (4)
2 sites
G A A T T C C T T A A G
HindIII  (10)
1 site
A A G C T T T T C G A A
SacII  (103)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
PvuII  (818)
1 site
C A G C T G G T C G A C
MscI  (875)
1 site
T G G C C A A C C G G T
BbsI  (1061)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AfeI  (1127)
1 site
A G C G C T T C G C G A
BclI  (1255)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (1370)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
AlwNI  (1450)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (1450)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PshAI  (1511)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
SnaBI  (1537)
1 site
T A C G T A A T G C A T
Bpu10I  (1617)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
ApaLI  (1746)
1 site
G T G C A C C A C G T G
XmnI  (1818)
1 site
G A A N N N N T T C C T T N N N N A A G
araC
2566 .. 3444  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
2566 .. 3444  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
1631 .. 2491  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   1631 .. 1699  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1631 .. 2491  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   1700 .. 2491  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1631 .. 2491  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
pBBR1 Rep
872 .. 1534  =  663 bp
220 amino acids  =  24.4 kDa
Product: replication protein from the broad host
range plasmid pBBR1
pBBR1 Rep
872 .. 1534  =  663 bp
220 amino acids  =  24.4 kDa
Product: replication protein from the broad host
range plasmid pBBR1
araBAD promoter
3590 .. 3755  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
araBAD promoter
3590 .. 3755  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
soxR terminator
60 .. 96  =  37 bp
bidirectional E. coli soxR transcription terminator
soxR terminator
60 .. 96  =  37 bp
bidirectional E. coli soxR transcription terminator
tonB terminator
2507 .. 2538  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
2507 .. 2538  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
MCS
3776 .. 15  =  24 bp
multiple cloning site
MCS
3776 .. 15  =  24 bp
multiple cloning site
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