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pSMART® FOS v2.0 (linearized)

Linearized and dephosphorylated fosmid vector for transcription-free cloning with inducible amplification of copy number.

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pSMART FOS v2.0 (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Lucigen
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AhdI (7573) NotI (7561) SL1 Primer (7513 .. 7533) NspI (7256) BstZ17I (7239) SacII (6602) FseI (6598) BciVI - MreI (6587) XcmI (6397) AanI (6157) AanI (5961) StuI (5909) XbaI (5887) EcoNI (5613) SmaI (5590) TspMI - XmaI (5588) FspAI (5331) BsrGI (5299) SbfI (5062) BlpI (4601) AfeI (4517) MfeI (4092) NdeI (4076) KpnI (3876) AhdI (8) NotI (15) SwaI (50) SR4 Primer (51 .. 71) ScaI (851) AvrII (881) SalI (1416) HpaI (1453) BstEII (1473) SexAI * (1477) PspOMI (2111) ApaI (2115) BsaI (2269) PfoI * (2327) AleI (2540) PvuI (3212) BclI * (3281) BseRI (3385) SnaBI (3452) Acc65I (3872) pSMART® FOS v2.0 7581 bp
AhdI  (7573)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7561)
2 sites
G C G G C C G C C G C C G G C G
NspI  (7256)
1 site
R C A T G Y Y G T A C R
BstZ17I  (7239)
1 site
G T A T A C C A T A T G
SacII  (6602)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
FseI  (6598)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BciVI  (6587)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
MreI  (6587)
1 site
C G C C G G C G G C G G C C G C
XcmI  (6397)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
AanI  (6157)
2 sites
T T A T A A A A T A T T
AanI  (5961)
2 sites
T T A T A A A A T A T T
StuI  (5909)
1 site
A G G C C T T C C G G A
XbaI  (5887)
1 site
T C T A G A A G A T C T
EcoNI  (5613)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (5590)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (5588)
1 site
C C C G G G G G G C C C
XmaI  (5588)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
FspAI  (5331)
1 site
R T G C G C A Y Y A C G C G T R
BsrGI  (5299)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SbfI  (5062)
1 site
C C T G C A G G G G A C G T C C
BlpI  (4601)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (4517)
1 site
A G C G C T T C G C G A
MfeI  (4092)
1 site
C A A T T G G T T A A C
NdeI  (4076)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
KpnI  (3876)
1 site
G G T A C C C C A T G G
AhdI  (8)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (15)
2 sites
G C G G C C G C C G C C G G C G