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Plasmid Files

pSMART® FOS v2.0 (linearized)

Linearized and dephosphorylated fosmid vector for transcription-free cloning with inducible amplification of copy number.

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pSMART FOS v2.0 (linearized) Sequence and MappSMART FOS v2.0 (linearized).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 < PmlI > (7581) AhdI (7573) NotI (7561) SL1 Primer (7513 .. 7533) NspI (7256) BstZ17I (7239) SacII (6602) FseI (6598) BciVI - MreI (6587) XcmI (6397) AanI (6157) AanI (5961) StuI (5909) XbaI (5887) EcoNI (5613) SmaI (5590) TspMI - XmaI (5588) FspAI (5331) BsrGI (5299) SbfI (5062) BlpI (4601) AfeI (4517) MfeI (4092) NdeI (4076) KpnI (3876) < PmlI > (0) AhdI (8) NotI (15) SwaI (50) SR4 Primer (51 .. 71) ScaI (851) AvrII (881) SalI (1416) HpaI (1453) BstEII (1473) SexAI * (1477) PspOMI (2111) ApaI (2115) BsaI (2269) PfoI * (2327) AleI (2540) PvuI (3212) BclI * (3281) BseRI (3385) SnaBI (3452) Acc65I (3872) pSMART® FOS v2.0 7581 bp
End  (7581)
0 sites
AhdI  (7573)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7561)
2 sites
G C G G C C G C C G C C G G C G
NspI  (7256)
1 site
R C A T G Y Y G T A C R
BstZ17I  (7239)
1 site
G T A T A C C A T A T G
SacII  (6602)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
FseI  (6598)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BciVI  (6587)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
MreI  (6587)
1 site
C G C C G G C G G C G G C C G C
XcmI  (6397)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
AanI  (6157)
2 sites
T T A T A A A A T A T T
AanI  (5961)
2 sites
T T A T A A A A T A T T
StuI  (5909)
1 site
A G G C C T T C C G G A
XbaI  (5887)
1 site
T C T A G A A G A T C T
EcoNI  (5613)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (5590)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (5588)
1 site
C C C G G G G G G C C C
XmaI  (5588)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
FspAI  (5331)
1 site
R T G C G C A Y Y A C G C G T R
BsrGI  (5299)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SbfI  (5062)
1 site
C C T G C A G G G G A C G T C C
BlpI  (4601)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (4517)
1 site
A G C G C T T C G C G A
MfeI  (4092)
1 site
C A A T T G G T T A A C
NdeI  (4076)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
KpnI  (3876)
1 site
G G T A C C C C A T G G
Start  (0)
0 sites
AhdI  (8)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (15)
2 sites
G C G G C C G C C G C C G G C G
SwaI  (50)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
ScaI  (851)
1 site
A G T A C T T C A T G A
AvrII  (881)
1 site
C C T A G G G G A T C C
SalI  (1416)
1 site
G T C G A C C A G C T G
HpaI  (1453)
1 site
G T T A A C C A A T T G
BstEII  (1473)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
SexAI  (1477)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
PspOMI  (2111)
1 site
G G G C C C C C C G G G
ApaI  (2115)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BsaI  (2269)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2327)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
AleI  (2540)
1 site
C A C N N N N G T G G T G N N N N C A C
PvuI  (3212)
1 site
C G A T C G G C T A G C
BclI  (3281)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (3385)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SnaBI  (3452)
1 site
T A C G T A A T G C A T
Acc65I  (3872)
1 site
G G T A C C C C A T G G
SL1 Primer
21-mer  /  57% GC
1 binding site
7513 .. 7533  =  21 annealed bases
Tm  =  59°C
SR4 Primer
21-mer  /  33% GC
1 binding site
51 .. 71  =  21 annealed bases
Tm  =  52°C
sopA
3178 .. 4353  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
3178 .. 4353  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
repE
4932 .. 5687  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
4932 .. 5687  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
CmR
221 .. 880  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
221 .. 880  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6073 .. 6687  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6073 .. 6687  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1661 .. 2134  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
1661 .. 2134  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
1002 .. 1400  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
cos
1002 .. 1400  =  399 bp
lambda cos site; allows packaging into phage
lambda particles
incC
4679 .. 4929  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4679 .. 4929  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5778 .. 5997  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5778 .. 5997  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
cat promoter
118 .. 220  =  103 bp
promoter of the E. coli cat gene
cat promoter
118 .. 220  =  103 bp
promoter of the E. coli cat gene
rrnB T1 terminator
69 .. 114  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
69 .. 114  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
tonB terminator
894 .. 925  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
894 .. 925  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
T7Te terminator
7454 .. 7481  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7454 .. 7481  =  28 bp
phage T7 early transcription terminator
T7 promoter
28 .. 46  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
28 .. 46  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7540 .. 7558  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7540 .. 7558  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2207 .. 3178  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
2207 .. 3178  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
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