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pALTER®-MAX

Mammalian expression vector with the CMV enhancer/promoter and with ampicillin and chloramphenicol resistance markers.

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pALTER-MAX.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BglII (5533) AlwNI (5112) AhdI (4633) XmnI (4033) EcoO109I (3653) PfoI (3594) BspEI (2832) BsrGI (96) SpeI (152) NdeI (387) SnaBI (493) AsiSI (664) Eco53kI (727) SacI (729) I-PpoI (851) BfuAI - BspMI (877) BbsI (961) PaeR7I - XhoI (1091) EcoRI (1096) AflIII - MluI (1102) Acc65I (1108) KpnI (1112) XbaI (1114) SalI (1120) AccI (1121) TspMI - XmaI (1125) SmaI (1127) EagI - NotI (1131) HpaI (1301) MfeI (1310) NgoMIV (1612) NaeI (1614) DraIII (1720) SexAI * (2123) SfiI (2309) BseRI (2352) StuI (2355) AvrII (2356) Bpu10I (2609) pALTER®-MAX 5538 bp
BglII  (5533)
1 site
A G A T C T T C T A G A
AlwNI  (5112)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (4633)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
XmnI  (4033)
1 site
G A A N N N N T T C C T T N N N N A A G
EcoO109I  (3653)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3594)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BspEI  (2832)
1 site
T C C G G A A G G C C T
BsrGI  (96)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (152)
1 site
A C T A G T T G A T C A
NdeI  (387)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (493)
1 site
T A C G T A A T G C A T
AsiSI  (664)
1 site
G C G A T C G C C G C T A G C G
Eco53kI  (727)
1 site
G A G C T C C T C G A G
SacI  (729)
1 site
G A G C T C C T C G A G
I-PpoI  (851)
1 site
C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G

I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
BfuAI  (877)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (877)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BbsI  (961)
1 site
G