Resources
Plasmid Files

pCMV-MIR

Vector for expressing microRNA (miRNA) precursors using the CMV promoter, with a GFP reporter for transfection and a neomycin resistance marker.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCMV-MIR.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer


AanI - PsiI (6136) SexAI * (5530) SfiI (5352) StuI (5303) BspDI * - ClaI * (5282) PflFI - Tth111I (5005) AclI (4418) KpnI (4002) Acc65I (3998) BmgBI (3900) PstI (3283) BsrGI (301) SpeI (357) AseI (365) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) SalI (985) AccI (986) BamHI (992) AsiSI - SgfI (1023) MreI - SgrAI (1025) AscI - BssHII (1029) NheI (1054) BmtI (1058) RsrII (1060) MluI (1066) BsiWI (1069) NotI (1075) PaeR7I - PspXI - XhoI (1081) EcoRV (1125) PmeI (1159) SacII (1173) TspMI - XmaI (1199) SmaI (1201) AleI (1259) XL39 (reverse primer) (1265 .. 1284) AhdI (1315) BbvCI - Bpu10I (1485) BsmBI (1549) AgeI (1747) pCMV-MIR 6219 bp
AanI  (6136)
1 site
T T A T A A A A T A T T
PsiI  (6136)
1 site
T T A T A A A A T A T T
SexAI  (5530)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (5352)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (5303)
1 site
A G G C C T T C C G G A
BspDI  (5282)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (5282)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PflFI  (5005)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5005)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AclI  (4418)
1 site
A A C G T T T T G C A A
KpnI  (4002)
1 site
G G T A C C C C A T G G
Acc65I  (3998)
1 site
G G T A C C C C A T G G
BmgBI  (3900)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PstI  (3283)
1 site
C T G C A G G A C G T C
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (357)
1 site
A C T A G T T G A T C A
AseI  (365)
1 site
A T T A A T T A A T T A
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C